論文 - 詳細
| RRC ID | 1604 |
|---|---|
| 著者 | Saitoh O, Mizutori Y, Takamura N, Yamasaki H, Kita A, Kuwahara H, Nagayama Y. |
| タイトル | Adenovirus-mediated gene delivery of interleukin-10, but not transforming growth factor beta, ameliorates the induction of Graves' hyperthyroidism in BALB/c mice. |
| ジャーナル | Clin Exp Immunol |
| Abstract |
Interleukin-10 (IL-10) and transforming growth factor-beta (TGF-beta) are well known anti-inflammatory cytokines. We have studied the effect of adenovirus-mediated IL-10 and TGF-beta gene delivery on the induction of Graves' hyperthyroidism in our mouse model that involves repeated injections of adenovirus expressing the thyrotropin receptor A subunit (AdTSHR). We first constructed adenoviruses encoding the two cytokines (AdIL10 and AdTGF(beta)) and confirmed expression by in vitro infection of COS cells. Susceptible BALB/c mice were injected twice with AdTSHR alone or together with AdIL10 or AdTGF(beta), and bled two weeks after the second immunization. Significantly elevated serum thyroxine levels were seen in 26% of mice immunized with AdTSHR and AdIL10 versus 61% with AdTSHR alone. Levels of thyroid stimulating antibody, but not nonstimulating antibody, were also decreased, and TSHR-specific splenocyte secretion of interferon-gamma in recall assays was impaired in mice treated with AdIL10. In contrast, AdTGF(beta) had little effect on hyperthyroidism. Overall, our findings demonstrate that gene delivery of IL-10, but not TGF-beta, suppresses the induction of Graves' hyperthyroidism in a mouse model. However, the effect of IL-10 is less powerful than we observed previously with T helper type 2-inducers including adenovirus expressing IL-4, Shistosoma mansoni infection or alpha-galactosylceramide. |
| 巻・号 | 141(3) |
| ページ | 405-11 |
| 公開日 | 2005-9-1 |
| DOI | 10.1111/j.1365-2249.2005.02874.x |
| PII | CEI2874 |
| PMID | 16045729 |
| PMC | PMC1809459 |
| MeSH | Adenoviridae / genetics Animals Antibodies / immunology Autoantibodies / immunology* Female Genetic Vectors / administration & dosage Graves Disease / immunology* Immunoglobulins, Thyroid-Stimulating / immunology Interleukin-10 / genetics Interleukin-10 / immunology* Mice Mice, Inbred BALB C Models, Animal Protein Binding Receptors, Thyrotropin / immunology Thyroid Gland / immunology Transduction, Genetic / methods Transforming Growth Factor beta / genetics Transforming Growth Factor beta / immunology* |
| IF | 3.532 |
| 引用数 | 10 |
| WOS 分野 | IMMUNOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| 遺伝子材料 | pBluescript SKII+mIL10 (RDB01476) |