RRC ID 16040
著者 Kameda T, Kojima K, Zhang Q, Sezutsu H.
タイトル Identification of hornet silk gene with a characteristic repetitive sequence in Vespa simillima xanthoptera.
ジャーナル Comp Biochem Physiol B Biochem Mol Biol
Abstract Vssilk 5 is a gene encoding a component protein of the silk produced by the larvae of the yellow hornet (Vespa simillima, Vespinae, Vespidae). In this study, we deduced the complete cDNA sequence of Vssilk 5. It was found that 2 silk proteins, Vssilk 5 N and Vssilk 5 C, in the cocoon of the yellow hornet are both encoded by the Vssilk 5 gene. Vssilk 5 N and 5 C are the N- and C-terminal regions, respectively, of the Vssilk 5 pro-protein (Vssilk 5p). The complete amino acid sequences of Vssilk 5 N and Vssilk 5 C were deduced. Although a non-repetitive amino acid sequence and coiled-coil structure are properties common to the major components of silk proteins produced by the larvae of the social superfamilies Apoidea and Vespoidea of the Apocrita, nearly the entire sequence of Vssilk 5 C consisted of a repeated sequence of amino acids, and the calculated coiled-coil probability for this protein was low. Vssilk 5 N is a protein without a repetitive amino acid sequence and has a low coiled-coil probability. Moreover, we found a water soluble protein, Vssilk 5S that is likely segmented from Vssilk 5 C and contains an N-terminal sequence identical to that of Vssilk 5 C.
巻・号 161(1)
ページ 17-24
公開日 2012-1-1
DOI 10.1016/j.cbpb.2011.09.002
PII S1096-4959(11)00177-1
PMID 21925617
MeSH Amino Acid Sequence Amino Acids / metabolism Animals Electrophoresis, Polyacrylamide Gel Genes, Insect / genetics* Hydrophobic and Hydrophilic Interactions Molecular Sequence Data Protein Structure, Secondary Repetitive Sequences, Nucleic Acid / genetics* Silk / chemistry Silk / genetics* Wasps / genetics*
IF 2.219
引用数 6
WOS 分野 ZOOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY
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