| RRC ID |
16046
|
| 著者 |
Kawaoka S, Kadota K, Arai Y, Suzuki Y, Fujii T, Abe H, Yasukochi Y, Mita K, Sugano S, Shimizu K, Tomari Y, Shimada T, Katsuma S.
|
| タイトル |
The silkworm W chromosome is a source of female-enriched piRNAs.
|
| ジャーナル |
RNA
|
| Abstract |
In the silkworm, Bombyx mori, the W chromosome plays a dominant role in female determination. However, neither protein-coding genes nor transcripts have so far been isolated from the W chromosome. Instead, a large amount of functional transposable elements and their remnants are accumulated on the W chromosome. PIWI-interacting RNAs (piRNAs) are 23-30-nt-long small RNAs that potentially act as sequence-specific guides for PIWI proteins to silence transposon activity in animal gonads. In this study, by comparing ovary- and testis-derived piRNAs, we identified numerous female-enriched piRNAs. Our data indicated that female-enriched piRNAs are derived from the W chromosome. Moreover, comparative analyses on piRNA profiles from a series of W chromosome mutant strains revealed a striking enrichment of a specific set of transposon-derived piRNAs in the putative sex-determining region. Collectively, we revealed the nature of the silkworm W chromosome as a source of piRNAs.
|
| 巻・号 |
17(12)
|
| ページ |
2144-51
|
| 公開日 |
2011-12-1
|
| DOI |
10.1261/rna.027565.111
|
| PII |
rna.027565.111
|
| PMID |
22020973
|
| PMC |
PMC3222127
|
| MeSH |
Animals
Bombyx / genetics*
Bombyx / metabolism
Chromosomes, Insect / genetics*
DNA Transposable Elements
Female
Gene Expression Regulation
Gonads / metabolism
Male
Models, Genetic
RNA, Small Interfering / genetics*
RNA, Small Interfering / metabolism
Sex Characteristics
Sex Chromosomes / genetics*
Sex Determination Processes
|
| IF |
4.32
|
| 引用数 |
29
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| カイコ |
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