論文 - 詳細
| RRC ID | 16048 |
|---|---|
| 著者 | Kawaoka S, Arai Y, Kadota K, Suzuki Y, Hara K, Sugano S, Shimizu K, Tomari Y, Shimada T, Katsuma S. |
| タイトル | Zygotic amplification of secondary piRNAs during silkworm embryogenesis. |
| ジャーナル | RNA |
| Abstract |
PIWI-interacting RNAs (piRNAs) are 23-30-nucleotide-long small RNAs that act as sequence-specific silencers of transposable elements in animal gonads. In flies, genetics and deep sequencing data have led to a hypothesis for piRNA biogenesis called the ping-pong cycle, where antisense primary piRNAs initiate an amplification loop to generate sense secondary piRNAs. However, to date, the process of the ping-pong cycle has never been monitored at work. Here, by large-scale profiling of piRNAs from silkworm ovary and embryos of different developmental stages, we demonstrate that maternally inherited antisense-biased piRNAs trigger acute amplification of secondary sense piRNA production in zygotes, at a time coinciding with zygotic transcription of sense transposon mRNAs. These results provide on-site evidence for the ping-pong cycle. |
| 巻・号 | 17(7) |
| ページ | 1401-7 |
| 公開日 | 2011-7-1 |
| DOI | 10.1261/rna.2709411 |
| PII | rna.2709411 |
| PMID | 21628432 |
| PMC | PMC3138575 |
| MeSH | Animals Bombyx / embryology* Bombyx / genetics* Cluster Analysis Embryo, Nonmammalian Embryonic Development / genetics Embryonic Development / physiology Female Gene Amplification / physiology* Gene Expression Profiling Gene Expression Regulation, Developmental Microarray Analysis Models, Biological Molecular Sequence Data RNA, Messenger, Stored / analysis RNA, Messenger, Stored / genetics RNA, Messenger, Stored / metabolism RNA, Small Interfering / genetics* RNA, Small Interfering / metabolism Zygote / metabolism* |
| IF | 4.32 |
| 引用数 | 32 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
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