| RRC ID |
16048
|
| 著者 |
Kawaoka S, Arai Y, Kadota K, Suzuki Y, Hara K, Sugano S, Shimizu K, Tomari Y, Shimada T, Katsuma S.
|
| タイトル |
Zygotic amplification of secondary piRNAs during silkworm embryogenesis.
|
| ジャーナル |
RNA
|
| Abstract |
PIWI-interacting RNAs (piRNAs) are 23-30-nucleotide-long small RNAs that act as sequence-specific silencers of transposable elements in animal gonads. In flies, genetics and deep sequencing data have led to a hypothesis for piRNA biogenesis called the ping-pong cycle, where antisense primary piRNAs initiate an amplification loop to generate sense secondary piRNAs. However, to date, the process of the ping-pong cycle has never been monitored at work. Here, by large-scale profiling of piRNAs from silkworm ovary and embryos of different developmental stages, we demonstrate that maternally inherited antisense-biased piRNAs trigger acute amplification of secondary sense piRNA production in zygotes, at a time coinciding with zygotic transcription of sense transposon mRNAs. These results provide on-site evidence for the ping-pong cycle.
|
| 巻・号 |
17(7)
|
| ページ |
1401-7
|
| 公開日 |
2011-7-1
|
| DOI |
10.1261/rna.2709411
|
| PII |
rna.2709411
|
| PMID |
21628432
|
| PMC |
PMC3138575
|
| MeSH |
Animals
Bombyx / embryology*
Bombyx / genetics*
Cluster Analysis
Embryo, Nonmammalian
Embryonic Development / genetics
Embryonic Development / physiology
Female
Gene Amplification / physiology*
Gene Expression Profiling
Gene Expression Regulation, Developmental
Microarray Analysis
Models, Biological
Molecular Sequence Data
RNA, Messenger, Stored / analysis
RNA, Messenger, Stored / genetics
RNA, Messenger, Stored / metabolism
RNA, Small Interfering / genetics*
RNA, Small Interfering / metabolism
Zygote / metabolism*
|
| IF |
4.32
|
| 引用数 |
32
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| カイコ |
|
