| RRC ID |
1604
|
| 著者 |
Saitoh O, Mizutori Y, Takamura N, Yamasaki H, Kita A, Kuwahara H, Nagayama Y.
|
| タイトル |
Adenovirus-mediated gene delivery of interleukin-10, but not transforming growth factor beta, ameliorates the induction of Graves' hyperthyroidism in BALB/c mice.
|
| ジャーナル |
Clin Exp Immunol
|
| Abstract |
Interleukin-10 (IL-10) and transforming growth factor-beta (TGF-beta) are well known anti-inflammatory cytokines. We have studied the effect of adenovirus-mediated IL-10 and TGF-beta gene delivery on the induction of Graves' hyperthyroidism in our mouse model that involves repeated injections of adenovirus expressing the thyrotropin receptor A subunit (AdTSHR). We first constructed adenoviruses encoding the two cytokines (AdIL10 and AdTGF(beta)) and confirmed expression by in vitro infection of COS cells. Susceptible BALB/c mice were injected twice with AdTSHR alone or together with AdIL10 or AdTGF(beta), and bled two weeks after the second immunization. Significantly elevated serum thyroxine levels were seen in 26% of mice immunized with AdTSHR and AdIL10 versus 61% with AdTSHR alone. Levels of thyroid stimulating antibody, but not nonstimulating antibody, were also decreased, and TSHR-specific splenocyte secretion of interferon-gamma in recall assays was impaired in mice treated with AdIL10. In contrast, AdTGF(beta) had little effect on hyperthyroidism. Overall, our findings demonstrate that gene delivery of IL-10, but not TGF-beta, suppresses the induction of Graves' hyperthyroidism in a mouse model. However, the effect of IL-10 is less powerful than we observed previously with T helper type 2-inducers including adenovirus expressing IL-4, Shistosoma mansoni infection or alpha-galactosylceramide.
|
| 巻・号 |
141(3)
|
| ページ |
405-11
|
| 公開日 |
2005-9-1
|
| DOI |
10.1111/j.1365-2249.2005.02874.x
|
| PII |
CEI2874
|
| PMID |
16045729
|
| PMC |
PMC1809459
|
| MeSH |
Adenoviridae / genetics
Animals
Antibodies / immunology
Autoantibodies / immunology*
Female
Genetic Vectors / administration & dosage
Graves Disease / immunology*
Immunoglobulins, Thyroid-Stimulating / immunology
Interleukin-10 / genetics
Interleukin-10 / immunology*
Mice
Mice, Inbred BALB C
Models, Animal
Protein Binding
Receptors, Thyrotropin / immunology
Thyroid Gland / immunology
Transduction, Genetic / methods
Transforming Growth Factor beta / genetics
Transforming Growth Factor beta / immunology*
|
| IF |
3.532
|
| 引用数 |
10
|
|
WOS 分野
|
IMMUNOLOGY
|
| リソース情報 |
| 遺伝子材料 |
pBluescript SKII+mIL10 (RDB01476) |
