Reference - Detail
|Author||Gerdes SY, Scholle MD, Campbell JW, Balázsi G, Ravasz E, Daugherty MD, Somera AL, Kyrpides NC, Anderson I, Gelfand MS, Bhattacharya A, Kapatral V, D'Souza M, Baev MV, Grechkin Y, Mseeh F, Fonstein MY, Overbeek R, Barabási AL, Oltvai ZN, Osterman AL.|
|Title||Experimental determination and system level analysis of essential genes in Escherichia coli MG1655.|
Defining the gene products that play an essential role in an organism's functional repertoire is vital to understanding the system level organization of living cells. We used a genetic footprinting technique for a genome-wide assessment of genes required for robust aerobic growth of Escherichia coli in rich media. We identified 620 genes as essential and 3,126 genes as dispensable for growth under these conditions. Functional context analysis of these data allows individual functional assignments to be refined. Evolutionary context analysis demonstrates a significant tendency of essential E. coli genes to be preserved throughout the bacterial kingdom. Projection of these data over metabolic subsystems reveals topologic modules with essential and evolutionarily preserved enzymes with reduced capacity for error tolerance.
|MeSH||Aerobiosis Amino Acids / biosynthesis Culture Media DNA Footprinting / methods* DNA Transposable Elements Escherichia coli / genetics Escherichia coli / growth & development* Escherichia coli Proteins / genetics* Escherichia coli Proteins / metabolism Evolution, Molecular Gene Expression Regulation, Bacterial Genes, Essential Genome, Bacterial* Mutagenesis, Insertional Phylogeny|