RRC ID 1697
Author Jiang S, Ramachandran S.
Title Identification and molecular characterization of myosin gene family in Oryza sativa genome.
Journal Plant Cell Physiol.
Abstract Myosins play an important role in various developmental processes in plants. We have identified 14 myosin genes in rice (Oryza sativa cv. Nipponbare) genome using sequence information available in public databases. Phylogenetic analysis of these sequences with other plant and non-plant myosins revealed that two of the predicted sequences belonged to class VIII and the others to class XI. All of these genes were distributed on seven chromosomes in the rice genome. Domain searches on these sequences indicated that a typical rice myosin consisted of Myosin_N, head domain, neck (IQ motifs), tail, and dilute (DIL) domain. Based on the sequence information obtained from predicted myosins, we isolated and sequenced two full-length cDNAs, OsMyoVIIIA and OsMyoXIE, representing each of the two classes of myosins. These two cDNAs isolated from different organs existed in isoforms due to differential splicing and showed minor differences from the predicted myosin in exon organization. Out of 14 myosin genes 11 were expressed in three major organs: leaves, panicles, and roots, among which three myosins exhibited different expression levels. On the other hand, three of the total myosin sequences showed organ-specific expression. The existence of different myosin genes and their isoforms in different organs or tissues indicates the diversity of myosin functions in rice.
Volume 45(5)
Pages 590-9
Published 2004-5
PMID 15169941
MeSH Chromosome Mapping DNA, Complementary / analysis DNA, Complementary / genetics Databases, Genetic Evolution, Molecular Gene Expression Regulation, Plant / genetics Genome, Plant* Molecular Sequence Data Multigene Family* Myosins / genetics* Myosins / isolation & purification Myosins / metabolism Oryza / genetics* Oryza / metabolism Phylogeny Plant Leaves / genetics Plant Leaves / metabolism Plant Proteins / genetics* Plant Proteins / isolation & purification Plant Proteins / metabolism Plant Roots / genetics Plant Roots / metabolism Protein Isoforms / genetics Protein Isoforms / isolation & purification Protein Isoforms / metabolism Protein Structure, Tertiary / genetics Sequence Homology, Nucleic Acid
IF 3.929
Times Cited 30