論文 - 詳細
| RRC ID | 17395 |
|---|---|
| 著者 | Wu JC, Go AC, Samson M, Cintra T, Mirsoian S, Wu TF, Jow MM, Routman EJ, Chu DS. |
| タイトル | Sperm development and motility are regulated by PP1 phosphatases in Caenorhabditis elegans. |
| ジャーナル | Genetics |
| Abstract |
Sperm from different species have evolved distinctive motility structures, including tubulin-based flagella in mammals and major sperm protein (MSP)-based pseudopods in nematodes. Despite such divergence, we show that sperm-specific PP1 phosphatases, which are required for male fertility in mouse, function in multiple processes in the development and motility of Caenorhabditis elegans amoeboid sperm. We used live-imaging analysis to show the PP1 phosphatases GSP-3 and GSP-4 (GSP-3/4) are required to partition chromosomes during sperm meiosis. Postmeiosis, tracking fluorescently labeled sperm revealed that both male and hermaphrodite sperm lacking GSP-3/4 are immotile. Genetic and in vitro activation assays show lack of GSP-3/4 causes defects in pseudopod development and the rate of pseudopodial treadmilling. Further, GSP-3/4 are required for the localization dynamics of MSP. GSP-3/4 shift localization in concert with MSP from fibrous bodies that sequester MSP at the base of the pseudopod, where directed MSP disassembly facilitates pseudopod contraction. Consistent with a role for GSP-3/4 as a spatial regulator of MSP disassembly, MSP is mislocalized in sperm lacking GSP-3/4. Although a requirement for PP1 phosphatases in nematode and mammalian sperm suggests evolutionary conservation, we show PP1s have independently evolved sperm-specific paralogs in separate lineages. Thus PP1 phosphatases are highly adaptable and employed across a broad range of sexually reproducing species to regulate male fertility. |
| 巻・号 | 190(1) |
| ページ | 143-57 |
| 公開日 | 2012-1-1 |
| DOI | 10.1534/genetics.111.135376 |
| PII | genetics.111.135376 |
| PMID | 22042574 |
| PMC | PMC3249365 |
| MeSH | Animals Biological Evolution Caenorhabditis elegans / enzymology* Caenorhabditis elegans / genetics Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Chromosome Segregation / physiology Fertility / genetics Male Meiosis / physiology Models, Biological Phylogeny Protein Phosphatase 1 / genetics Protein Phosphatase 1 / metabolism* Pseudopodia / genetics Pseudopodia / metabolism Sperm Motility* Spermatogenesis / genetics Spermatozoa / enzymology* |
| IF | 4.015 |
| 引用数 | 23 |
| WOS 分野 | GENETICS & HEREDITY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm1647 |