RRC ID 17395
Author Wu JC, Go AC, Samson M, Cintra T, Mirsoian S, Wu TF, Jow MM, Routman EJ, Chu DS.
Title Sperm development and motility are regulated by PP1 phosphatases in Caenorhabditis elegans.
Journal Genetics
Abstract Sperm from different species have evolved distinctive motility structures, including tubulin-based flagella in mammals and major sperm protein (MSP)-based pseudopods in nematodes. Despite such divergence, we show that sperm-specific PP1 phosphatases, which are required for male fertility in mouse, function in multiple processes in the development and motility of Caenorhabditis elegans amoeboid sperm. We used live-imaging analysis to show the PP1 phosphatases GSP-3 and GSP-4 (GSP-3/4) are required to partition chromosomes during sperm meiosis. Postmeiosis, tracking fluorescently labeled sperm revealed that both male and hermaphrodite sperm lacking GSP-3/4 are immotile. Genetic and in vitro activation assays show lack of GSP-3/4 causes defects in pseudopod development and the rate of pseudopodial treadmilling. Further, GSP-3/4 are required for the localization dynamics of MSP. GSP-3/4 shift localization in concert with MSP from fibrous bodies that sequester MSP at the base of the pseudopod, where directed MSP disassembly facilitates pseudopod contraction. Consistent with a role for GSP-3/4 as a spatial regulator of MSP disassembly, MSP is mislocalized in sperm lacking GSP-3/4. Although a requirement for PP1 phosphatases in nematode and mammalian sperm suggests evolutionary conservation, we show PP1s have independently evolved sperm-specific paralogs in separate lineages. Thus PP1 phosphatases are highly adaptable and employed across a broad range of sexually reproducing species to regulate male fertility.
Volume 190(1)
Pages 143-57
Published 2012-1-1
DOI 10.1534/genetics.111.135376
PII genetics.111.135376
PMID 22042574
PMC PMC3249365
MeSH Animals Biological Evolution Caenorhabditis elegans / enzymology* Caenorhabditis elegans / genetics Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Chromosome Segregation / physiology Fertility / genetics Male Meiosis / physiology Models, Biological Phylogeny Protein Phosphatase 1 / genetics Protein Phosphatase 1 / metabolism* Pseudopodia / genetics Pseudopodia / metabolism Sperm Motility* Spermatogenesis / genetics Spermatozoa / enzymology*
IF 4.015
Times Cited 23
WOS Category GENETICS & HEREDITY
Resource
C.elegans tm1647