論文 - 詳細
| RRC ID | 17399 |
|---|---|
| 著者 | Hattori K, Matsui H. |
| タイトル | Diversity of fumarate reducing bacteria in the bovine rumen revealed by culture dependent and independent approaches. |
| ジャーナル | Anaerobe |
| Abstract |
Diversity of fumarate reducing (dissimilating) bacteria in the bovine rumen was analyzed by both culture dependent and independent methodologies. A total of 39 strains were isolated by using three different media and belonged to three different phyla (Proteobacteria, Fusobacteria, and Firmicutes). A primer set that amplified the fumarate reductase gene (frdA) from Proteobacteria was developed and two frdA clone libraries were constructed. Identities of deduced amino acid sequences of cloned frdA amplicons against known sequences ranged from 58% to 85% suggesting the presence of unknown fumarate reducing bacteria. This is the first report on the diversity of fumarate reducing bacteria in the rumen. |
| 巻・号 | 14(2) |
| ページ | 87-93 |
| 公開日 | 2008-4-1 |
| DOI | 10.1016/j.anaerobe.2007.12.002 |
| PII | S1075-9964(07)00103-5 |
| PMID | 18276172 |
| MeSH | Animals Bacteria / classification* Bacteria / genetics Bacteria / isolation & purification Bacteria / metabolism* Bacterial Proteins / genetics Biodiversity* Cattle DNA, Bacterial / genetics Fumarates / metabolism* Fusobacteria / classification Fusobacteria / genetics Fusobacteria / isolation & purification Fusobacteria / metabolism Gram-Positive Bacteria / classification Gram-Positive Bacteria / genetics Gram-Positive Bacteria / isolation & purification Gram-Positive Bacteria / metabolism Molecular Sequence Data Oxidation-Reduction Phylogeny Proteobacteria / classification Proteobacteria / genetics Proteobacteria / isolation & purification Proteobacteria / metabolism Rumen / microbiology* Sequence Analysis, DNA Sequence Homology, Amino Acid Succinate Dehydrogenase / genetics |
| IF | 2.709 |
| 引用数 | 17 |
| WOS 分野 | MICROBIOLOGY |
| リソース情報 | |
| 一般微生物 | JCM 6582 |