RRC ID 17409
著者 Iinuma K, Sato T, Akimoto N, Noguchi N, Sasatsu M, Nishijima S, Kurokawa I, Ito A.
タイトル Involvement of Propionibacterium acnes in the augmentation of lipogenesis in hamster sebaceous glands in vivo and in vitro.
ジャーナル J Invest Dermatol
Abstract Propionibacterium acnes is considered to be involved in the aggravation of acne vulgaris, but it remains unclear whether P. acnes directly influences lipogenesis in sebaceous glands. In this study, we showed that a culture medium of P. acnes (acnes-CM) and formalin-killed P. acnes (F-acnes) prepared from P. acnes strains, JCM6473 and JCM6425, intracellularly augmented lipid droplet formation and triacylglycerol (TG) synthesis in undifferentiated and insulin-differentiated hamster sebocytes. Acnes-CM and F-acnes prepared from four clinical P. acnes strains elicited the same lipogenesis augmentation. The augmented TG production resulted from an increase in the diacylglycerol acyltransferase activity. Topical application of acnes-CM to the skin of hamster auricles every day for 4 weeks revealed that sebum accumulation was augmented in sebaceous glands and ducts. Furthermore, both acnes-CM and F-acnes increased the production of 15-deoxy-Delta(12,14)-prostaglandin J(2) (15d-PGJ(2)), a cytochrome P450 (CYP)-linked sebaceous lipogenic factor, in differentiated sebocytes. A CYP inhibitor, SKF-525A, decreased the acnes-CM- and F-acnes-augmented production of TG and 15d-PGJ(2). Thus, to our knowledge these results provide previously unreported evidence that P. acnes directly participates in the augmentation of sebaceous lipogenesis through a proposed mechanism in which an increase of 15d-PGJ(2) production through the CYP pathway is closely associated with the enhancement of TG production.
巻・号 129(9)
ページ 2113-9
公開日 2009-9-1
DOI 10.1038/jid.2009.46
PII S0022-202X(15)34473-0
PMID 19282842
MeSH Acne Vulgaris / etiology Animals Cells, Cultured Cricetinae Lipogenesis* Male Mesocricetus Propionibacterium acnes / pathogenicity* Prostaglandin D2 / analogs & derivatives Prostaglandin D2 / physiology Sebaceous Glands / cytology Sebaceous Glands / metabolism* Sebum / physiology Triglycerides / biosynthesis
IF 7.143
引用数 42
WOS 分野 DERMATOLOGY
リソース情報
一般微生物 JCM 6425 JCM 6473