RRC ID 17421
Author Sasaki-Imamura T, Yano A, Yoshida Y.
Title Production of indole from L-tryptophan and effects of these compounds on biofilm formation by Fusobacterium nucleatum ATCC 25586.
Journal Appl Environ Microbiol
Abstract The l-tryptophan degradation product indole is a purported extracellular signaling molecule that influences biofilm formation in various bacteria. Here we analyzed the mechanisms of indole production in Fusobacterium nucleatum and the effects of tryptophan and indole on F. nucleatum planktonic and biofilm cells. The amino acid sequence deduced from the fn1943 gene in F. nucleatum ATCC 25586 was 28% identical to that deduced from tnaA in Escherichia coli, which encodes tryptophanase catalyzing the beta-elimination of l-tryptophan to produce indole. The fn1943 gene was cotranscribed with the downstream gene fn1944, which is a homolog of tnaB encoding low-affinity tryptophan permease. The transcript started at position -68 or -153 from the first nucleotide of the fn1943 translation initiation codon. Real-time quantitative PCR showed that much more F. nucleatum fn1943 transcripts were obtained from log-phase cells than from stationary-phase cells. Indole production by the purified recombinant protein encoded by fn1943 was examined using high-performance liquid chromatography. The K(m) and k(cat) of the enzyme were 0.26 +/- 0.03 mM and 0.74 +/- 0.04 s(-1), respectively. F. nucleatum biofilm formation and the biofilm supernatant concentration of indole increased dose dependently with increasing tryptophan concentrations. Exogenous indole also increased F. nucleatum biofilm formation in a dose-dependent manner. Even at very high concentrations, tryptophan did not affect fn1943 expression, whereas similar indole concentrations decreased expression. Thus, exogenous tryptophan and indole were suggested to increase F. nucleatum biofilms.
Volume 76(13)
Pages 4260-8
Published 2010-7-1
DOI 10.1128/AEM.00166-10
PII AEM.00166-10
PMID 20472741
PMC PMC2897440
MeSH Base Sequence Biofilms / drug effects* Biofilms / growth & development Fusobacterium nucleatum / drug effects* Fusobacterium nucleatum / enzymology Fusobacterium nucleatum / growth & development* Indoles / metabolism Indoles / pharmacology* Molecular Sequence Data Plankton / drug effects Plankton / growth & development Recombinant Proteins / genetics Recombinant Proteins / metabolism Tryptophan / metabolism* Tryptophan / pharmacology Tryptophanase / genetics Tryptophanase / metabolism
IF 4.016
Times Cited 40
General Microbes JCM 8532