論文 - 詳細
| RRC ID | 17422 |
|---|---|
| 著者 | Yamamoto S, Wada J, Katayama T, Jikimoto T, Nakamura M, Kinoshita S, Lee KM, Kawabata M, Shirakawa T. |
| タイトル | Genetically modified Bifidobacterium displaying Salmonella-antigen protects mice from lethal challenge of Salmonella Typhimurium in a murine typhoid fever model. |
| ジャーナル | Vaccine |
| Abstract |
We developed a novel vaccine platform utilizing Bifidobacterium as an antigen delivery vehicle for mucosal immunization. Genetically modified Bifidobacterium longum displaying Salmonella-flagellin on the cell surface was constructed for the oral typhoid vaccine. The efficiency of this vaccine was evaluated in a murine model of typhoid fever. We then orally administered 2.5 × 10(7) CFU of the recombinant Bifidobacterium longum (vaccine) or parental Bifidobacterium longum, or PBS to BALB/C mice every other day for 2 weeks. After the administration, a total of 42 mice (14 mice in each group) were challenged with Salmonella Typhimurium (1.0 × 10(7) CFU/mouse). While 12 mice in the PBS group, and 9 in the parental Bifidobacterium longum group died (median survival: 14 and 25 days), only two in the vaccine group died. These data support that our genetically modified Bifidobacterium antigen delivery system offers a promising vaccine platform for inducing efficient mucosal immunity. |
| 巻・号 | 28(41) |
| ページ | 6684-91 |
| 公開日 | 2010-9-24 |
| DOI | 10.1016/j.vaccine.2010.08.007 |
| PII | S0264-410X(10)01116-3 |
| PMID | 20709009 |
| MeSH | Animals Antibodies, Bacterial / immunology Antigens, Bacterial / immunology Bifidobacterium / immunology* Disease Models, Animal Female Flagellin / immunology Immunity, Mucosal* Mice Mice, Inbred BALB C Recombinant Fusion Proteins / immunology Salmonella typhimurium / immunology Typhoid Fever / immunology Typhoid Fever / prevention & control* Typhoid-Paratyphoid Vaccines / immunology* |
| IF | 3.143 |
| 引用数 | 21 |
| WOS 分野 | MEDICINE, RESEARCH & EXPERIMENTAL IMMUNOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 6 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM 1217 |