論文 - 詳細
| RRC ID | 17445 |
|---|---|
| 著者 | Yoshida Y, Ito S, Tamura H, Kunimatsu K. |
| タイトル | Use of a novel assay to evaluate enzymes that produce hydrogen sulfide in Fusobacterium nucleatum. |
| ジャーナル | J Microbiol Methods |
| Abstract |
To evaluate enzymes that produce hydrogen sulfide (H(2)S) in species of Fusobacterium nucleatum, we developed an assay based on SDS-polyacrylamide gel electrophoresis with renaturation followed by active staining. This assay provided precise insight into the enzymes that produce H(2)S in terms of their number and molecular weights. |
| 巻・号 | 80(3) |
| ページ | 313-5 |
| 公開日 | 2010-3-1 |
| DOI | 10.1016/j.mimet.2010.01.015 |
| PII | S0167-7012(10)00017-5 |
| PMID | 20093145 |
| MeSH | Electrophoresis, Polyacrylamide Gel / methods Fusobacterium Infections / complications Fusobacterium Infections / enzymology* Fusobacterium Infections / microbiology* Fusobacterium nucleatum / chemistry Fusobacterium nucleatum / metabolism* Humans Hydrogen Sulfide / metabolism* Molecular Weight Periodontal Diseases / enzymology Periodontal Diseases / etiology Species Specificity Staining and Labeling |
| IF | 1.707 |
| 引用数 | 7 |
| WOS 分野 | BIOCHEMICAL RESEARCH METHODS MICROBIOLOGY |
| リソース情報 | |
| 一般微生物 | JCM 8532 JCM 11023 JCM 11024 JCM 11025 JCM 12990 |