論文 - 詳細
RRC ID | 17452 |
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著者 | Arakawa K, Kawai Y, Ito Y, Nakamura K, Chujo T, Nishimura J, Kitazawa H, Saito T. |
タイトル | HPLC purification and re-evaluation of chemical identity of two circular bacteriocins, gassericin A and reutericin 6. |
ジャーナル | Lett Appl Microbiol |
Abstract |
AIM:The study aimed for the complete purification and recharacterization of the highly hydrophobic circular bacteriocins, gassericin A and reutericin 6. METHODS AND RESULTS:Gassericin A and reutericin 6 were purified to homogeneity using previously described method and reverse-phase HPLC with an octyl column and eluents of aqueous acetonitrile and 2-propanol. Mass analysis, N-terminal sequencing and bacteriocin assay of the HPLC-purified bacteriocins showed the two bacteriocins had identical seamless circular structures with the same m/z value (5651) of [M + H](+) and both had the same specific activity. D/L-amino acid composition analysis using two distinct methods with the chiral fluorescent derivatization reagents (+)-1-(9-fluorenyl)ethyl chloroformate and O-phthalaldehyde/N-acetyl-L-cystein revealed neither gassericin A nor reutericin 6 contained D-alanine residues contrary to our previous results. CONCLUSION:Purified gassericin A and reutericin 6 are chemically identical circular molecules containing no D-alanine residues. SIGNIFICANCE AND IMPACT OF THE STUDY:The HPLC conditions developed in this study will facilitate advanced purification and correct characterization of other highly hydrophobic bacteriocins. |
巻・号 | 50(4) |
ページ | 406-11 |
公開日 | 2010-4-1 |
DOI | 10.1111/j.1472-765X.2010.02810.x |
PII | LAM2810 |
PMID | 20156306 |
MeSH | Bacteriocins / chemistry* Bacteriocins / isolation & purification* Chromatography, High Pressure Liquid* Lactobacillus / chemistry Mass Spectrometry |
IF | 2.173 |
引用数 | 21 |
WOS 分野 | BIOTECHNOLOGY & APPLIED MICROBIOLOGY MICROBIOLOGY |
リソース情報 | |
一般微生物 | JCM 1002 JCM 1131 JCM 11657 |