| RRC ID |
17454
|
| Author |
Sakai T, Oishi K, Asahara T, Takada T, Yuki N, Matsumoto K, Nomoto K, Kushiro A.
|
| Title |
M-RTLV agar, a novel selective medium to distinguish Lactobacillus casei and Lactobacillus paracasei from Lactobacillus rhamnosus.
|
| Journal |
Int J Food Microbiol
|
| Abstract |
We developed a novel selective medium, modified-rhamnose-2,3,5-triphenyltetrazolium chloride-LBS-vancomycin agar (M-RTLV agar), that utilizes the fermentability of L-rhamnose to distinguish Lactobacillus casei and Lactobacillus paracasei from Lactobacillus rhamnosus. Whereas L. casei and L. paracasei formed red colonies on the M-RTLV agar, L. rhamnosus formed either pink-toned colonies or white colonies with a red spot. An intervention study was conducted to confirm the capability of M-RTLV agar to detect ingested L. casei when recovered from human feces. Subjects consumed one bottle daily of a fermented milk product (Yakult or Yakult Light, which contains L. casei strain Shirota; LcS) for 7 days. Diluents of the fecal samples were cultivated on M-RTLV agar. We were able to enumerate circular medium-sized red colonies, which were morphologically similar to L. casei/L. paracasei but clearly distinguishable from the remaining colonies owing to the color difference. These colonies were then subjected to enzyme-linked immunosorbent assay in order to identify the LcS. The viable counts of LcS were 6.6+/-0.7 log(10) CFU/g feces after intake of Yakult and 6.5+/-0.6 log(10) CFU/g feces after intake of Yakult Light (mean+/-SD).
|
| Volume |
139(3)
|
| Pages |
154-60
|
| Published |
2010-5-15
|
| DOI |
10.1016/j.ijfoodmicro.2010.03.019
|
| PII |
S0168-1605(10)00159-5
|
| PMID |
20385416
|
| MeSH |
Adult
Agar / chemistry*
Colony Count, Microbial
Culture Media / chemistry*
Cultured Milk Products / microbiology
Feces / microbiology
Female
Fermentation
Humans
Lacticaseibacillus casei / isolation & purification*
Lacticaseibacillus rhamnosus / isolation & purification*
Male
Middle Aged
Rhamnose / chemistry
Young Adult
|
| IF |
4.187
|
| Times Cited |
21
|
|
WOS Category
|
FOOD SCIENCE & TECHNOLOGY
MICROBIOLOGY
|
| Resource |
| General Microbes |
JCM 1112
JCM 1117
JCM 1152
JCM 1157 |
