Reference - Detail
| RRC ID | 17454 |
|---|---|
| Author | Sakai T, Oishi K, Asahara T, Takada T, Yuki N, Matsumoto K, Nomoto K, Kushiro A. |
| Title | M-RTLV agar, a novel selective medium to distinguish Lactobacillus casei and Lactobacillus paracasei from Lactobacillus rhamnosus. |
| Journal | Int J Food Microbiol |
| Abstract |
We developed a novel selective medium, modified-rhamnose-2,3,5-triphenyltetrazolium chloride-LBS-vancomycin agar (M-RTLV agar), that utilizes the fermentability of L-rhamnose to distinguish Lactobacillus casei and Lactobacillus paracasei from Lactobacillus rhamnosus. Whereas L. casei and L. paracasei formed red colonies on the M-RTLV agar, L. rhamnosus formed either pink-toned colonies or white colonies with a red spot. An intervention study was conducted to confirm the capability of M-RTLV agar to detect ingested L. casei when recovered from human feces. Subjects consumed one bottle daily of a fermented milk product (Yakult or Yakult Light, which contains L. casei strain Shirota; LcS) for 7 days. Diluents of the fecal samples were cultivated on M-RTLV agar. We were able to enumerate circular medium-sized red colonies, which were morphologically similar to L. casei/L. paracasei but clearly distinguishable from the remaining colonies owing to the color difference. These colonies were then subjected to enzyme-linked immunosorbent assay in order to identify the LcS. The viable counts of LcS were 6.6+/-0.7 log(10) CFU/g feces after intake of Yakult and 6.5+/-0.6 log(10) CFU/g feces after intake of Yakult Light (mean+/-SD). |
| Volume | 139(3) |
| Pages | 154-60 |
| Published | 2010-5-15 |
| DOI | 10.1016/j.ijfoodmicro.2010.03.019 |
| PII | S0168-1605(10)00159-5 |
| PMID | 20385416 |
| MeSH | Adult Agar / chemistry* Colony Count, Microbial Culture Media / chemistry* Cultured Milk Products / microbiology Feces / microbiology Female Fermentation Humans Lacticaseibacillus casei / isolation & purification* Lacticaseibacillus rhamnosus / isolation & purification* Male Middle Aged Rhamnose / chemistry Young Adult |
| IF | 4.187 |
| Times Cited | 21 |
| WOS Category | FOOD SCIENCE & TECHNOLOGY MICROBIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| Total number of mentions | 0 |
| Resource | |
| General Microbes | JCM 1112 JCM 1117 JCM 1152 JCM 1157 |