RRC ID 17470
著者 Whon TW, Jung MJ, Roh SW, Nam YD, Park EJ, Shin KS, Bae JW.
タイトル Oceanobacillus kimchii sp. nov. isolated from a traditional Korean fermented food.
ジャーナル J Microbiol
Abstract A moderate halophile, strain X50(T), was isolated from mustard kimchi, a traditional Korean fermented food. The organism grew under conditions ranging from 0-15.0% (w/v) NaCl (optimum: 3.0%), pH 7.0-10.0 (optimum: pH 9.0) and 15-45°C (optimum: 37°C). The morphological, physiological, and biochemical features and the 16S rRNA gene sequences of strain X50(T) were characterized. Colonies of the isolate were creamcolored and the cells were rod-shaped. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain X50(T) belongs to the genus Oceanobacillus and is closely related phylogenetically to the type strain O. iheyensis HTE831(T) (98.9%) and O. oncorhynchi subsp. oncorhynchi R-2(T) (97.0%). The cellular fatty acid profiles predominately included anteiso-C(15:0) and iso-C(15:0). The G+C content of the genomic DNA of the isolate was 37.9 mol% and the major isoprenoid quinone was MK-7. Analysis of the 16S rRNA gene sequences, DNA-DNA relatedness and physiological and biochemical tests indicated genotypic and phenotypic differences among strain X50(T) and reference species in the genus Oceanobacillus. Therefore, strain X50(T) was proposed as a novel species and named Oceanobacillus kimchii. The type strain of the new species is X50(T) (=JCM 16803(T) =KACC 14914(T) =DSM 23341(T)).
巻・号 48(6)
ページ 862-6
公開日 2010-12-1
DOI 10.1007/s12275-010-0214-7
PMID 21221947
MeSH Bacillaceae / classification* Bacillaceae / genetics Bacillaceae / isolation & purification* Base Composition Cluster Analysis DNA, Bacterial / chemistry DNA, Bacterial / genetics DNA, Ribosomal / chemistry DNA, Ribosomal / genetics Fatty Acids / analysis Food Microbiology* Hydrogen-Ion Concentration Molecular Sequence Data Nucleic Acid Hybridization Phylogeny RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA Sodium Chloride / metabolism Temperature
IF 2.845
引用数 22
WOS 分野 MICROBIOLOGY
リソース情報
一般微生物 JCM 16803