| RRC ID |
17495
|
| Author |
Inoue J, Nishiumi S, Fujishima Y, Masuda A, Shiomi H, Yamamoto K, Nishida M, Azuma T, Yoshida M.
|
| Title |
Autophagy in the intestinal epithelium regulates Citrobacter rodentium infection.
|
| Journal |
Arch Biochem Biophys
|
| Abstract |
Autophagy, a ubiquitous degradation pathway, is important for the survival and homeostasis of cells. Previous studies have demonstrated the role of autophagy in host defense against bacterial infection, but the importance of autophagy in the intestinal epithelium for the regulation of bacterial infection has not been fully elucidated. In this study, we showed that the essential autophagy protein Atg7 is required for resistance to Citrobacter rodentium infection in the intestinal epithelium. Infected mice in which Atg7 had been conditionally deleted from the intestinal epithelium exhibited greater clinical evidence of disease and higher expression levels of pro-inflammatory cytokine mRNA in the large intestine. Moreover, C. rodentium clearance was reduced in the Atg7 conditional knockout mice. These results demonstrate that autophagy in intestinal epithelial cells plays an important role in host defense against C. rodentium infection and the regulation of C. rodentium infectious colitis.
|
| Volume |
521(1-2)
|
| Pages |
95-101
|
| Published |
2012-5-1
|
| DOI |
10.1016/j.abb.2012.03.019
|
| PII |
S0003-9861(12)00110-5
|
| PMID |
22475450
|
| MeSH |
Animals
Antibodies, Bacterial / blood
Autophagy*
Autophagy-Related Protein 7
Base Sequence
Citrobacter rodentium* / immunology
Citrobacter rodentium* / pathogenicity
Colitis / genetics
Colitis / immunology
Colitis / microbiology
Colitis / pathology
Cytokines / genetics
DNA Primers / genetics
Enterobacteriaceae Infections / genetics
Enterobacteriaceae Infections / immunology
Enterobacteriaceae Infections / microbiology*
Enterobacteriaceae Infections / pathology*
Immunoglobulin G / blood
Intestinal Mucosa / immunology
Intestinal Mucosa / microbiology*
Intestinal Mucosa / pathology*
Mice
Mice, Knockout
Microtubule-Associated Proteins / deficiency
Microtubule-Associated Proteins / genetics
Microtubule-Associated Proteins / physiology
RNA, Messenger / genetics
RNA, Messenger / metabolism
|
| IF |
3.391
|
| Times Cited |
25
|
|
WOS Category
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Mice |
RBRC00806 |
