RRC ID 17560
Author Geffeney SL, Cueva JG, Glauser DA, Doll JC, Lee TH, Montoya M, Karania S, Garakani AM, Pruitt BL, Goodman MB.
Title DEG/ENaC but not TRP channels are the major mechanoelectrical transduction channels in a C. elegans nociceptor.
Journal Neuron
Abstract Many nociceptors detect mechanical cues, but the ion channels responsible for mechanotransduction in these sensory neurons remain obscure. Using in vivo recordings and genetic dissection, we identified the DEG/ENaC protein, DEG-1, as the major mechanotransduction channel in ASH, a polymodal nociceptor in Caenorhabditis elegans. But DEG-1 is not the only mechanotransduction channel in ASH: loss of deg-1 revealed a minor current whose properties differ from those expected of DEG/ENaC channels. This current was independent of two TRPV channels expressed in ASH. Although loss of these TRPV channels inhibits behavioral responses to noxious stimuli, we found that both mechanoreceptor currents and potentials were essentially wild-type in TRPV mutants. We propose that ASH nociceptors rely on two genetically distinct mechanotransduction channels and that TRPV channels contribute to encoding and transmitting information. Because mammalian and insect nociceptors also coexpress DEG/ENaCs and TRPVs, the cellular functions elaborated here for these ion channels may be conserved.
Volume 71(5)
Pages 845-57
Published 2011-9-8
DOI 10.1016/j.neuron.2011.06.038
PII S0896-6273(11)00596-4
PMID 21903078
PMC PMC3170654
MeSH Amiloride / pharmacology Animals Animals, Genetically Modified Behavior, Animal / physiology Biophysical Phenomena / drug effects Biophysical Phenomena / genetics Biophysical Phenomena / physiology* Caenorhabditis elegans Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / physiology* Electric Stimulation / methods Mechanotransduction, Cellular / genetics Mechanotransduction, Cellular / physiology* Membrane Potentials / drug effects Membrane Potentials / genetics* Membrane Proteins / genetics Membrane Proteins / physiology* Mutation, Missense / genetics Nociceptors / metabolism* Patch-Clamp Techniques / methods Reaction Time / drug effects Reaction Time / genetics Sodium / metabolism Sodium Channel Blockers / pharmacology TRPC Cation Channels / metabolism* Touch / physiology
IF 14.319
Times Cited 42
WOS Category NEUROSCIENCES
Resource
C.elegans unc-8(tm2701)