RRC ID 17565
Author Asakawa K, Toyoshima KE, Ishibashi N, Tobe H, Iwadate A, Kanayama T, Hasegawa T, Nakao K, Toki H, Noguchi S, Ogawa M, Sato A, Tsuji T.
Title Hair organ regeneration via the bioengineered hair follicular unit transplantation.
Journal Sci Rep
Abstract Organ regenerative therapy aims to reproduce fully functional organs to replace organs that have been lost or damaged as a result of disease, injury, or aging. For the fully functional regeneration of ectodermal organs, a concept has been proposed in which a bioengineered organ is developed by reproducing the embryonic processes of organogenesis. Here, we show that a bioengineered hair follicle germ, which was reconstituted with embryonic skin-derived epithelial and mesenchymal cells and ectopically transplanted, was able to develop histologically correct hair follicles. The bioengineered hair follicles properly connected to the host skin epithelium by intracutaneous transplantation and reproduced the stem cell niche and hair cycles. The bioengineered hair follicles also autonomously connected with nerves and the arrector pili muscle at the permanent region and exhibited piloerection ability. Our findings indicate that the bioengineered hair follicles could restore physiological hair functions and could be applicable to surgical treatments for alopecia.
Volume 2
Pages 424
Published 2012
DOI 10.1038/srep00424
PMID 22645640
PMC PMC3361021
MeSH Alopecia / surgery Animals Bioengineering / methods* Cells, Cultured Epithelial Cells / cytology Epithelial Cells / metabolism Green Fluorescent Proteins / genetics Green Fluorescent Proteins / metabolism Hair / cytology Hair / physiology* Hair Follicle / cytology Hair Follicle / metabolism Hair Follicle / transplantation* Humans Mesenchymal Stromal Cells / cytology Mesenchymal Stromal Cells / metabolism Mice Mice, Inbred C57BL Mice, Transgenic Microscopy, Confocal Regeneration* Skin Transplantation / methods Stem Cells / cytology Stem Cells / metabolism Tissue Engineering / methods
IF 4.011
Times Cited 17
WOS Category DERMATOLOGY
Resource
Mice C57BL/6-TgN(act-EGFP)OsbC14-Y01-FM131 (RBRC00267)