論文 - 詳細
| RRC ID | 17618 |
|---|---|
| 著者 | Tomita Y, Imai K, Senju S, Irie A, Inoue M, Hayashida Y, Shiraishi K, Mori T, Daigo Y, Tsunoda T, Ito T, Nomori H, Nakamura Y, Kohrogi H, Nishimura Y. |
| タイトル | A novel tumor-associated antigen, cell division cycle 45-like can induce cytotoxic T-lymphocytes reactive to tumor cells. |
| ジャーナル | Cancer Sci |
| Abstract |
The present study attempted to identify a useful tumor-associated antigen (TAA) for lung cancer immunotherapy and potential immunogenic peptides derived from the TAA. We focused on cell division cycle 45-like (CDC45L), which has a critical role in the initiation and elongation steps of DNA replication, as a novel candidate TAA for immunotherapy based on a genome-wide cDNA microarray analysis of lung cancer. The CDC45L was overexpressed in the majority of lung cancer tissues, but not in the adjacent non-cancerous tissues or in many normal adult tissues. We examined the in vitro and in vivo anti-tumor effects of cytotoxic T-lymphocytes (CTL) specific to CDC45L-derived peptides induced from HLA-A24 (A*24:02)-positive donors. We identified three CDC45L-derived peptides that could reproducibly induce CDC45L-specific and HLA-A24-restricted CTL from both healthy donors and lung cancer patients. The CTL could effectively lyse lung cancer cells that endogenously expressed both CDC45L and HLA-A24. In addition, we found that CDC45L (556) KFLDALISL(564) was eminent in that it induced not only HLA-A24 but also HLA-A2 (A*02:01)-restricted antigen specific CTL. Furthermore, the adoptive transfer of the CDC45L-specific CTL inhibited the growth of human cancer cells engrafted into immunocompromised mice. These results suggest that these three CDC45L-derived peptides are highly immunogenic epitopes and CDC45L is a novel TAA that might be a useful target for lung cancer immunotherapy. |
| 巻・号 | 102(4) |
| ページ | 697-705 |
| 公開日 | 2011-4-1 |
| DOI | 10.1111/j.1349-7006.2011.01865.x |
| PMID | 21231984 |
| MeSH | Animals Biomarkers, Tumor / genetics Biomarkers, Tumor / metabolism Blotting, Northern Blotting, Western Cell Cycle Proteins / genetics Cell Cycle Proteins / immunology Cell Cycle Proteins / metabolism* Epitopes / immunology* Gene Expression Profiling HLA-A Antigens / immunology* HLA-A Antigens / metabolism HLA-A24 Antigen Humans Immunoenzyme Techniques Mice Mice, Inbred NOD Mice, SCID Neoplasms / immunology* Neoplasms / metabolism Neoplasms / therapy* Oligonucleotide Array Sequence Analysis Peptide Fragments / immunology* Peptide Fragments / metabolism RNA, Messenger / genetics Reverse Transcriptase Polymerase Chain Reaction T-Lymphocytes, Cytotoxic / immunology* Tumor Cells, Cultured |
| IF | 4.966 |
| 引用数 | 25 |
| WOS 分野 | ONCOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 6 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | PANC-1(RCB2095) T2(RCB1932) |