RRC ID 17633
Author Yamada P, Nemoto M, Shigemori H, Yokota S, Isoda H.
Title Isolation of 5-(hydroxymethyl)furfural from Lycium chinense and its inhibitory effect on the chemical mediator release by basophilic cells.
Journal Planta Med
Abstract The effect of hot water extracts of LYCIUM CHINENSE fruits (LCF) on the β-hexosaminidase (β-hexo) release by IgE sensitized BSA stimulated rat basophilic leukemia (RBL-2H3) cells was investigated. The ethylacetate (EtOAc) layer of the extract has shown an inhibitory effect on β-hexo release from RBL-2H3 cells at the antigen antibody binding stage. The water (H₂O) fraction (EFW) of the chloroform (CHCl₃) extract from the EtOAc layer also inhibited β-hexo release at the same stage in a dose-dependent manner. With column chromatography preparation, proton and carbon nuclear magnetic resonance (¹H and ¹³C NMR) spectra, electron ionization mass spectrometer (EI-MS) spectra, and high-performance liquid chromatography (HPLC) analysis, the active component was determined to be 5-(hydroxymethyl)furfural (5-HMF). Thus, the 5-HMF showed an inhibitory effect on β-hexo release at the antigen-antibody binding stage and the antibody-receptor binding stage. Furthermore, 5-HMF suppressed [Ca²+] I influx in the IgE-sensitized BSA-stimulated RBL-2H3 cells. Our results show that 5-HMF may be useful for the treatment or prevention of type I allergic diseases.
Volume 77(5)
Pages 434-40
Published 2011-3-1
DOI 10.1055/s-0030-1250402
PMID 21031336
MeSH Animals Anti-Allergic Agents / pharmacology* Antigen-Antibody Reactions Basophils / drug effects Basophils / enzymology Basophils / metabolism* Cell Line, Tumor Dose-Response Relationship, Drug Fruit / chemistry Furaldehyde / analogs & derivatives* Furaldehyde / isolation & purification Furaldehyde / pharmacology Immunoglobulin E / immunology Leukemia, Basophilic, Acute / metabolism Lycium / chemistry* Nuclear Magnetic Resonance, Biomolecular Plant Extracts / isolation & purification Plant Extracts / pharmacology* Rats beta-N-Acetylhexosaminidases / metabolism*
IF 2.687
Times Cited 24
Human and Animal Cells KU812(RCB0495)