論文 - 詳細
| RRC ID | 17635 |
|---|---|
| 著者 | Cao Y, Kimura S, Itoi T, Honda K, Ohtake H, Omasa T. |
| タイトル | Fluorescence in situ hybridization using bacterial artificial chromosome (BAC) clones for the analysis of chromosome rearrangement in Chinese hamster ovary cells. |
| ジャーナル | Methods |
| Abstract |
Chromosome identification using Chinese hamster ovary (CHO) genomic bacterial artificial chromosome (BAC) clones has the potential to contribute to the analysis and understanding of chromosomal instability of CHO cell lines and to improve our understanding of chromosome organization during the establishment of recombinant CHO cells. Fluorescence in situ hybridization imaging using BAC clones as probes (BAC-FISH) can provide valuable information for the identification of chromosomes. In this study, we identified chromosomes and analyzed the chromosome rearrangement in CHO cells using BAC-FISH methods. |
| 巻・号 | 56(3) |
| ページ | 418-23 |
| 公開日 | 2012-3-1 |
| DOI | 10.1016/j.ymeth.2011.11.002 |
| PII | S1046-2023(11)00234-9 |
| PMID | 22100493 |
| MeSH | Animals CHO Cells Chromosome Aberrations* Chromosomes, Artificial, Bacterial* Cricetinae Cricetulus In Situ Hybridization, Fluorescence / methods* |
| IF | 3.812 |
| 引用数 | 12 |
| WOS 分野 | BIOCHEMICAL RESEARCH METHODS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | CHO-K1(RCB0285) |