RRC ID 17635
Author Cao Y, Kimura S, Itoi T, Honda K, Ohtake H, Omasa T.
Title Fluorescence in situ hybridization using bacterial artificial chromosome (BAC) clones for the analysis of chromosome rearrangement in Chinese hamster ovary cells.
Journal Methods
Abstract Chromosome identification using Chinese hamster ovary (CHO) genomic bacterial artificial chromosome (BAC) clones has the potential to contribute to the analysis and understanding of chromosomal instability of CHO cell lines and to improve our understanding of chromosome organization during the establishment of recombinant CHO cells. Fluorescence in situ hybridization imaging using BAC clones as probes (BAC-FISH) can provide valuable information for the identification of chromosomes. In this study, we identified chromosomes and analyzed the chromosome rearrangement in CHO cells using BAC-FISH methods.
Volume 56(3)
Pages 418-23
Published 2012-3-1
DOI 10.1016/j.ymeth.2011.11.002
PII S1046-2023(11)00234-9
PMID 22100493
MeSH Animals CHO Cells Chromosome Aberrations* Chromosomes, Artificial, Bacterial* Cricetinae Cricetulus In Situ Hybridization, Fluorescence / methods*
IF 3.812
Times Cited 12
WOS Category BIOCHEMICAL RESEARCH METHODS BIOCHEMISTRY & MOLECULAR BIOLOGY
Resource
Human and Animal Cells CHO-K1(RCB0285)