論文 - 詳細
| RRC ID | 17642 |
|---|---|
| 著者 | Iwasaka K, Tomita K, Ozawa Y, Katayama T, Sakagami H. |
| タイトル | Effect of CO2 laser irradiation on hormesis induction in cultured oral cells. |
| ジャーナル | In Vivo |
| Abstract |
BACKGROUND:Many drugs (including toxicants) and radiation therapy have been reported to exert bi-phasic hormetic effects on cultured cells, but only when both the concentration and treatment time were optimal. Most previous studies have been carried out with multiple laser modalities other than CO(2) laser, and there has been no comparison of the hormetic response between normal and tumor cells. We investigated here whether CO(2) laser treatment induces hormesis in human gingival fibroblast (HGF) and oral squamous cell carcinoma (HSC-2) cells. MATERIALS AND METHODS:Cells were cultured for 24, 48 or 72 hours after exposure to various irradiation powers, and the viable cell number was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method. RESULTS:CO(2) laser irradiation stimulated cell growth at low and inhibited it at high irradiation power. Among three dispatch modes, super pulse (SP)2 most effectively induced growth stimulation in HGF, at an irradiation dose slightly lower than that which induced cytotoxicity. Higher irradiation doses were comparably cytotoxic against both normal (HGF) and tumor (HSC-2) cells, reaching a plateau of cytotoxicity within 24 hours. CONCLUSION:Since both the range and magnitude of hormetic response in HGF cells were very narrow and small, it is crucial to establish the optimal conditions for hormesis induction for clinical application in dentistry. |
| 巻・号 | 25(1) |
| ページ | 93-8 |
| 公開日 | 2011-1-1 |
| PII | 25/1/93 |
| PMID | 21282740 |
| MeSH | Cell Growth Processes / radiation effects* Cells, Cultured Dose-Response Relationship, Radiation Fibroblasts / cytology Fibroblasts / radiation effects Formazans Gingiva / cytology Gingiva / radiation effects* Humans Lasers, Gas / adverse effects* Mouth Mucosa / cytology Mouth Mucosa / radiation effects* Tetrazolium Salts |
| IF | 1.541 |
| 引用数 | 9 |
| WOS 分野 | MEDICINE, RESEARCH & EXPERIMENTAL |
| リソース情報 | |
| ヒト・動物細胞 | HSC-2(RCB1945) |