Reference - Detail
| RRC ID | 17654 |
|---|---|
| Author | Li D, Sakashita S, Morishita Y, Kano J, Shiba A, Sato T, Noguchi M. |
| Title | Binding of lactoferrin to IGBP1 triggers apoptosis in a lung adenocarcinoma cell line. |
| Journal | Anticancer Res |
| Abstract |
BACKGROUND:Lactoferrin (Lf), an iron-binding protein present in mammalian secretions, plays important roles in cancer prevention by inducing apoptosis. MATERIALS AND METHODS:PC-14 lung adenocarcinoma cells were exposed to bovine Lf (bLf) protein and the expression of caspase-3 and apoptosis protease-activating factor-1 (APAF-1) was assessed. To investigate the molecular mechanism of apoptosis induced by bLf, a major Lf-binding protein was screened using a protein microarray with bLf protein as the probe. Protein interaction was demonstrated by co-immunoprecipitation, immunofluorescence and phosphatase activity assay. RESULTS:Lf directly suppressed the proliferation of the PC-14 cells by triggering their apoptosis. Lf was shown to bind specifically with a 36-kDa protein, immunoglobulin (CD79A)-binding protein 1 (IGBP1). The binding complex interacted with the catalytic subunit of protein phosphatase 2A (PP2A), thus reducing the phosphatase activity of PP2A and triggering apoptosis. CONCLUSION:Lf binds IGBP1 and promotes the acceleration of cellular apoptosis. |
| Volume | 31(2) |
| Pages | 529-34 |
| Published | 2011-2-1 |
| PII | 31/2/529 |
| PMID | 21378334 |
| MeSH | Adaptor Proteins, Signal Transducing Adenocarcinoma / drug therapy Adenocarcinoma / metabolism Adenocarcinoma / pathology Adenocarcinoma of Lung Animals Apoptosis / drug effects* Apoptosis / physiology Cattle Cell Growth Processes / drug effects Cell Growth Processes / physiology Cell Line, Tumor Humans Intracellular Signaling Peptides and Proteins / metabolism* Lactoferrin / metabolism Lactoferrin / pharmacology* Lung Neoplasms / drug therapy Lung Neoplasms / metabolism Lung Neoplasms / pathology Microscopy, Fluorescence Molecular Chaperones Protein Array Analysis Reverse Transcriptase Polymerase Chain Reaction |
| IF | 1.994 |
| Times Cited | 20 |
| WOS Category | ONCOLOGY |
| Resource | |
| Human and Animal Cells | PC-14(RCB0446) |