RRC ID 17666
Author Nakagawa S, Arai Y, Kishida T, Hiraoka N, Tsuchida S, Inoue H, Sakai R, Mazda O, Kubo T.
Title Lansoprazole inhibits nitric oxide and prostaglandin E(2) production in murine macrophage RAW 264.7 cells.
Journal Inflammation
Abstract Aberrantly activated macrophages, which overproduce inflammatory mediators, are involved in the pathogenesis of many inflammatory diseases. We analyzed the anti-inflammatory activity of lansoprazole (LPZ), a typical proton pump (P-ATPase) inhibitor, on RAW264.7 murine macrophages. Treatment of lipopolysaccharide (LPS)-stimulated RAW264.7 cells with LPZ inhibited the production of nitric oxide (NO) and prostaglandin E(2) (PGE(2)). Since P-ATPase expression was not observed in RAW264.7 cells, the anti-inflammatory effect of LPZ was independent of ATPase. In contrast, diphenylene iodonium (DPI), an inhibitor of nicotinamide adenine dinucleotide phosphate (NADPH) oxidase, decreased NO but not PGE(2) levels. LPZ suppressed the LPS-stimulated production by RAW264.7 cells of reactive oxygen species (ROS), which plays an important role in inflammatory responses. ROS elevation in these cells was associated with NO but not PGE(2) production, suggesting that LPZ inhibits NO production by suppressing NADPH oxidase activity. These findings suggest that LPZ may be useful in the treatment of many inflammatory diseases associated with activated macrophages.
Volume 35(3)
Pages 1062-8
Published 2012-6
DOI 10.1007/s10753-011-9412-7
PMID 22134422
MeSH 2-Pyridinylmethylsulfinylbenzimidazoles / pharmacology* Animals Anti-Inflammatory Agents / pharmacology Cell Line Dinoprostone / biosynthesis* Enzyme Inhibitors / pharmacology Inflammation / drug therapy Inflammation / pathology Lansoprazole Lipopolysaccharides / immunology Macrophages / drug effects Macrophages / metabolism* Mice NADPH Oxidases / antagonists & inhibitors* Nitric Oxide / biosynthesis* Onium Compounds / pharmacology Proton Pump Inhibitors Reactive Oxygen Species / metabolism
IF 2.884
Times Cited 6
Human and Animal Cells RAW 264 (RCB0535)