RRC ID 17667
Author Tahara K, Sakai T, Yamamoto H, Takeuchi H, Hirashima N, Kawashima Y.
Title Improvements in transfection efficiency with chitosan modified poly(DL-lactide-co-glycolide) nanospheres prepared by the emulsion solvent diffusion method, for gene delivery.
Journal Chem. Pharm. Bull.
Abstract This study sought to evaluate the in vitro transfection efficiency of plasmid DNA (pDNA)-loaded chitosan-modified poly(DL-lactide-co-glycolide) nanospheres (CS-PLGA NS) in a gene-delivery system. Using the emulsion solvent diffusion (ESD) method, pDNA-loaded PLGA NS was prepared and the surface of the PLGA NS was modified by binding to CS. Gene transfection ability of CS-PLGA NS was examined in A549 cells. The luciferase gene was used as a reporter gene. The pattern of luciferase activity by pDNA-loaded CS-PLGA NS was initially weak, but gradually grew stronger before decreasing activity. These phenomena should be in accordance with the sustained-release profile of pDNA from PLGA NS in the cytosol and the pDNA protection against DNase. Positively charged CS-PLGA NS was found, by 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt (MTS) assay, not to exhibit cytotoxicity on A549 cells. These results suggest that CS-PLGA NS are potential contributors to efficient pDNA delivery due to their increased interactions with cells and lack of cytotoxic effects.
Volume 59(3)
Pages 298-301
Published 2011
DOI 10.1248/cpb.59.298
PII JST.JSTAGE/cpb/59.298
PMID 21372409
MeSH Cell Line, Tumor Chitosan / chemistry* Diffusion Emulsions / chemistry Humans Lactic Acid / administration & dosage Lactic Acid / chemistry* Lactic Acid / toxicity Nanospheres / chemistry* Plasmids / chemistry Plasmids / metabolism Polyglycolic Acid / administration & dosage Polyglycolic Acid / chemistry* Polyglycolic Acid / toxicity Polylactic Acid-Polyglycolic Acid Copolymer Solvents / chemistry Transfection / methods
IF 1.258
Times Cited 9
Human and Animal Cells A549 (RCB0098)