論文 - 詳細
| RRC ID | 17670 |
|---|---|
| 著者 | Tsunashima Y, Kondo A, Matsuda T, Togari A. |
| タイトル | Hydrocortisone inhibits cellular proliferation by downregulating hepatocyte growth factor synthesis in human osteoblasts. |
| ジャーナル | Biol Pharm Bull |
| Abstract |
Glucocorticoids have multiple systemic effects that may influence bone metabolism but also directly affect osteoblasts by decreasing their proliferation. Using human osteoblastic SaM-1 cells, we examined whether the effects of hydrocortisone on cellular proliferation are mediated by hepatocyte growth factor (HGF). Human osteoblasts constitutively express both HGF and c-Met, its receptor. Hydrocortisone decreased the gene and protein expression of HGF as well as proliferation in SaM-1 cells. These hydrocortisone (0.01-1 µM)-induced decreases in HGF synthesis and cellular proliferation occurred in a concentration-dependent manner. However, no hydrocortisone (0.01-1 µM)-induced decrease in cellular proliferation was observed in human osteosarcoma-derived cells (HOS and SaOS-2), which are not able to produce HGF. In the cellular proliferation in SaM-1 cells, the decrease was blocked concentration-dependently by exogenously applied HGF (0.01-3 ng/ml). Furthermore, SU11274 (1 µM), a highly specific inhibitor of c-Met, suppressed the proliferation of SaM-1 cells, but not HOS cells. From these results, we concluded that hydrocortisone inhibits the proliferation of SaM-1 cells by interrupting the autocrine/paracrine loop via the downregulation of HGF synthesis. |
| 巻・号 | 34(5) |
| ページ | 700-3 |
| 公開日 | 2011-1-1 |
| DOI | 10.1248/bpb.34.700 |
| PII | JST.JSTAGE/bpb/34.700 |
| PMID | 21532160 |
| MeSH | Base Sequence Cell Proliferation / drug effects* DNA Primers Down-Regulation / drug effects* Hepatocyte Growth Factor / biosynthesis* Humans Hydrocortisone / pharmacology* Osteoblasts / drug effects* Osteoblasts / metabolism Reverse Transcriptase Polymerase Chain Reaction |
| IF | 1.863 |
| 引用数 | 3 |
| WOS 分野 | PHARMACOLOGY & PHARMACY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | HOS(RCB0992) Saos-2(RCB0428) |