論文 - 詳細
| RRC ID | 17693 |
|---|---|
| 著者 | Amano K, Hata K, Muramatsu S, Wakabayashi M, Takigawa Y, Ono K, Nakanishi M, Takashima R, Kogo M, Matsuda A, Nishimura R, Yoneda T. |
| タイトル | Arid5a cooperates with Sox9 to stimulate chondrocyte-specific transcription. |
| ジャーナル | Mol Biol Cell |
| Abstract |
SRY-box-containing gene 9 (Sox9) is an essential transcription factor in chondrocyte lineage determination and differentiation. Recent studies demonstrated that Sox9 controls the transcription of chondrocyte-specific genes in association with several other transcriptional regulators. To further understand the molecular mechanisms by which Sox9 influences transcriptional events during chondrocyte differentiation, we attempted to identify transcriptional partners of Sox9 and to examine their roles in chondrocyte differentiation. We isolated AT-rich interactive domain-containing protein 5a (Arid5a; also known as Mrf1) as an activator of the Col2a1 gene promoter from an ATDC5 cDNA library. Arid5a was highly expressed in cartilage and induced during chondrocyte differentiation. Furthermore, Arid5a physically interacted with Sox9 in nuclei and up-regulated the chondrocyte-specific action of Sox9. Overexpression of Arid5a stimulated chondrocyte differentiation in vitro and in an organ culture system. In contrast, Arid5a knockdown inhibited Col2a1 expression in chondrocytes. In addition, Arid5a binds directly to the promoter region of the Col2a1 gene and stimulates acetylation of histone 3 in the region. Our results suggest that Arid5a may directly interact with Sox9 and thereby enhance its chondrocyte-specific action. |
| 巻・号 | 22(8) |
| ページ | 1300-11 |
| 公開日 | 2011-4-15 |
| DOI | 10.1091/mbc.E10-07-0566 |
| PII | mbc.E10-07-0566 |
| PMID | 21346191 |
| PMC | PMC3078073 |
| MeSH | Adenoviridae Animals Carrier Proteins / genetics Carrier Proteins / metabolism* Cartilage / embryology Cartilage / metabolism Cell Differentiation / genetics Cell Line Cell Proliferation Chondrocytes / cytology Chondrocytes / metabolism* Chondrogenesis / genetics Collagen Type II / genetics Collagen Type II / metabolism* Embryo, Mammalian / metabolism Escherichia coli Female Gene Expression Gene Library Intracellular Signaling Peptides and Proteins Luciferases, Firefly / analysis Mice Organ Culture Techniques Plasmids Promoter Regions, Genetic Protein Binding / genetics Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism* SOX9 Transcription Factor / genetics SOX9 Transcription Factor / metabolism* Transcriptional Activation Tripartite Motif Proteins |
| IF | 3.791 |
| 引用数 | 37 |
| WOS 分野 | CELL BIOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | ATDC5(RCB0565) |