RRC ID 1784
Author Oshima K, Takeda M, Kuranaga E, Ueda R, Aigaki T, Miura M, Hayashi S.
Title IKK epsilon regulates F actin assembly and interacts with Drosophila IAP1 in cellular morphogenesis.
Journal Curr. Biol.
Abstract Differentiated cells assume complex shapes through polarized cell migration and growth. These processes require the restricted organization of the actin cytoskeleton at limited subcellular regions. IKK epsilon is a member of the IkappaB kinase family, and its developmental role has not been clear. Drosophila IKK epsilon was localized to the ruffling membrane of cultured cells and was required for F actin turnover at the cell margin. In IKK epsilon mutants, tracheal terminal cells, bristles, and arista laterals, which require accurate F actin assembly for their polarized elongation, all exhibited aberrantly branched morphology. These phenotypes were sensitive to a change in the dosage of Drosophila inhibitor of apoptosis protein 1 (DIAP1) and the caspase DRONC without apparent change in cell viability. In contrast to this, hyperactivation of IKK epsilon destabilized F actin-based structures. Expression of a dominant-negative form of IKK epsilon increased the amount of DIAP1. The results suggest that at the physiological level, IKK epsilon acts as a negative regulator of F actin assembly and maintains the fidelity of polarized elongation during cell morphogenesis. This IKK epsilon function involves the negative regulation of the nonapoptotic activity of DIAP1.
Volume 16(15)
Pages 1531-7
Published 2006-8-8
DOI 10.1016/j.cub.2006.06.032
PII S0960-9822(06)01750-7
PMID 16887350
MeSH Actins / metabolism* Animals Apoptosis / physiology* Blotting, Western Cell Differentiation / physiology* Drosophila / physiology* Drosophila Proteins / metabolism* Genetic Testing Histocytochemistry I-kappa B Kinase / metabolism* Inhibitor of Apoptosis Proteins / metabolism* Microscopy, Fluorescence Morphogenesis / physiology* Trachea / cytology Trachea / growth & development
IF 9.251
Times Cited 65
WOS Category BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
Resource
DNA material pUAST-IKKepsilon (RDB08029) pUAST-IKKepsilonK41A (RDB08030)
Drosophila 2615R-1 2615R-2 12284R-2 8091R-1 8091R-2 DGRC#109662