論文 - 詳細
| RRC ID | 179 |
|---|---|
| 著者 | Nakano T, Kawamoto S, Lai CY, Hsu LW, Lin YC, Sasaki T, Aki T, Shigeta S, Goto T, Ohmori N, Sato S, Goto S, Ono K, Chen CL. |
| タイトル | Characterization of immunosuppressive factors expressed in serum by rat tolerogenic liver transplantation. |
| ジャーナル | Transplant Proc |
| Abstract |
BACKGROUND:In a rat tolerogenic model of orthotopic liver transplantation (OLT), recipient serum after OLT (post-OLT serum) possesses strong immunosuppressive activity. This study aimed to identify immunosuppressive factors present in early post-OLT serum. METHODS:Immunosuppressive activity was evaluated in vitro by inhibition of the mixed-lymphocyte reaction (MLR). Autoantigens recognized by MLR-inhibitory IgG were identified by the internal protein sequencing. RESULTS:Recipient post-OLT serum inhibited MLR, and OLT-inducible IgG was the major immunosuppressive factor. IgG from post-OLT sera (2 to 3 weeks) specifically reacted to 31; 34; and 73-kd autoantigens on spleen cells. The internal sequences of the 31- and 34-kd antigens coincided completely with those of histone H1 molecules. Immunodepletion of anti-histone H1 antibodies (Abs) from early post-OLT serum abolished the MLR-inhibitory activity. Furthermore, rabbit polyclonal Ab-directed histone H1 not only significantly suppressed rat and human MLR but also prolonged survival of heart allografts. Flow-cytometric analysis revealed that some live PVG splenocytes were stained with antihistone H1 Abs, and that these positive cells increased on Con A stimulation. Western blot analysis indicated that several cross-reactive antigens against anti-histone H1 Abs were found in their membrane fraction. CONCLUSIONS:In this study we provide evidence that autoreactive Abs, against histone H1 are a major OLT-induced graft survival factor, and may play at least a part in overcoming the acute rejection phase to establish solid allograft tolerance. |
| 巻・号 | 37(1) |
| ページ | 80-1 |
| 公開日 | 2005-1-1 |
| DOI | 10.1016/j.transproceed.2004.12.290 |
| PII | S0041-1345(04)01748-8 |
| PMID | 15808554 |
| MeSH | Animals Autoantigens / immunology Heart Transplantation / immunology* Histones / immunology Immunoglobulin G / blood Immunoglobulin G / immunology Immunosuppression Therapy / methods* Liver Transplantation / immunology* Lymphocyte Culture Test, Mixed Models, Animal Models, Immunological Rats Spleen / immunology Transplantation Tolerance / immunology* |
| IF | 0.784 |
| 引用数 | 13 |
| WOS 分野 | SURGERY TRANSPLANTATION IMMUNOLOGY |
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