Reference - Detail
|Author||Sasaki N, Sasamura T, Ishikawa HO, Kanai M, Ueda R, Saigo K, Matsuno K.|
|Title||Polarized exocytosis and transcytosis of Notch during its apical localization in Drosophila epithelial cells.|
Notch (N) and its ligands, Delta (Dl) and Serrate (Ser), are transmembrane proteins that mediate the cell-cell interactions necessary for many cell-fate decisions. In Drosophila, N is predominantly localized to the apical portion of epithelial cells, but the mechanisms and functions of this localization are unknown. Here, we found N, Dl, and Ser were mostly located in the region from the subapical complex (SAC) to the apical portion of the adherens junctions (AJs) in wing disc epithelium. N was delivered to the SAC/AJs in two phases. First, polarized exocytosis specifically delivered nascent N to the apical plasma membrane and AJs in an O-fut1-independent manner. Second, N at the plasma membrane was relocated to the SAC/AJs by Dynamin- and Rab5-dependent transcytosis; this step required the O-fut1 function. Disruption of the apical polarity by Drosophila E-cadherin (DEcad) knock down caused N and Dl localization to the SAC/AJs to fail. N, but not Dl, formed a specific complex with DEcad in vivo. Finally, our results suggest that juxtacrine signaling in epithelia generally depends on the apicobasally polarized structure of epithelial cells.
|MeSH||Adherens Junctions / metabolism Animals Cadherins / genetics Cadherins / metabolism Calcium-Binding Proteins / metabolism Cell Membrane / metabolism Cell Polarity / physiology* Drosophila / physiology* Drosophila Proteins / analysis Drosophila Proteins / genetics Drosophila Proteins / metabolism* Endocytosis Epithelial Cells / chemistry Epithelial Cells / metabolism* Epithelial Cells / ultrastructure Exocytosis / physiology* Fucosyltransferases / metabolism Image Processing, Computer-Assisted Immunohistochemistry Intercellular Signaling Peptides and Proteins / metabolism Intracellular Signaling Peptides and Proteins Jagged-1 Protein Ligands Membrane Proteins / metabolism Models, Biological Receptors, Notch / analysis Receptors, Notch / metabolism* Serrate-Jagged Proteins Signal Transduction|
|WOS Category||GENETICS & HEREDITY CELL BIOLOGY|