| RRC ID |
18085
|
| 著者 |
Ding ZC, Huang L, Blazar BR, Yagita H, Mellor AL, Munn DH, Zhou G.
|
| タイトル |
Polyfunctional CD4⁺ T cells are essential for eradicating advanced B-cell lymphoma after chemotherapy.
|
| ジャーナル |
Blood
|
| Abstract |
The finding that many chemotherapeutic agents have immunostimulatory effects has provided the impetus to combine chemotherapy and immunotherapy for synergistic antitumor effects. However, the critical determinants of effective antitumor immunity after chemotherapy have not been defined. Here we report that adoptive transfer of tumor-specific CD4⁺ T cells after chemotherapy with cyclophosphamide gave rise to polyfunctional CD4⁺ effector cells, which in turn intensified the inflammatory milieu and enhanced the activation of CD8⁺ T cells in the tumor microenvironment. Although this combined chemoimmunotherapy initially resulted in progressive regression of advanced B-cell lymphoma, its therapeutic efficacy was not durable and most mice succumbed to late relapse. Notably, relapse was associated with acquisition of a tolerized phenotype in tumor-specific CD4⁺ T cells, characterized by overexpression of program death-1 (PD-1). Remarkably, effective antitumor immunity was maintained and cure became prevalent when polyfunctional CD4⁺ effector cells were prevented from undergoing PD-1-mediated tolerization, either by antibody blockade of the PD-1-PD-L1 pathway, or targeted ablation of PD-1 in tumor-specific CD4⁺ T cells. Our study suggests that tumor-reactive CD4⁺ T cells act as the "gatekeepers" of the host antitumor immunity in the postchemotherapy setting, thereby their functional status governs the choice between eradication versus regrowth of residual tumors.
|
| 巻・号 |
120(11)
|
| ページ |
2229-39
|
| 公開日 |
2012-9-13
|
| DOI |
10.1182/blood-2011-12-398321
|
| PII |
S0006-4971(20)46404-3
|
| PMID |
22859605
|
| PMC |
PMC3447781
|
| MeSH |
Animals
Antibodies, Blocking
Antineoplastic Agents / therapeutic use
CD4 Antigens / metabolism
Cell Line, Tumor
Combined Modality Therapy
Cyclophosphamide / therapeutic use
Cytotoxicity, Immunologic
Female
Immunotherapy, Adoptive*
Lymphoma, B-Cell / drug therapy
Lymphoma, B-Cell / immunology*
Lymphoma, B-Cell / prevention & control
Lymphoma, B-Cell / therapy*
Mice
Mice, Inbred BALB C
Mice, Knockout
Mice, Transgenic
Neoplasm Transplantation
Programmed Cell Death 1 Receptor / antagonists & inhibitors
Programmed Cell Death 1 Receptor / genetics
Programmed Cell Death 1 Receptor / metabolism
Secondary Prevention
T-Lymphocytes, Helper-Inducer / immunology*
T-Lymphocytes, Helper-Inducer / metabolism
T-Lymphocytes, Helper-Inducer / transplantation*
Up-Regulation
|
| IF |
17.794
|
| 引用数 |
43
|
|
WOS 分野
|
HEMATOLOGY
|
| リソース情報 |
| 実験動物マウス |
RBRC00904 |
