RRC ID 18117
Author Ochiai K, Yoshikawa Y, Yoshimatsu K, Oonuma T, Tomioka Y, Takeda E, Arikawa J, Mominoki K, Omi T, Hashizume K, Morimatsu M.
Title Valine 1532 of human BRC repeat 4 plays an important role in the interaction between BRCA2 and RAD51.
Journal FEBS Lett
Abstract The breast cancer susceptibility protein BRCA2 is essential for recombinational DNA repair. BRCA2 specifically binds to RAD51 via eight BRC repeat motifs and delivers RAD51 to double-stranded DNA breaks. In this study, a mammalian two-hybrid assay and competitive ELISA showed that the interaction between BRC repeat 4 (BRC4) and RAD51 was strengthened by the substitution of a single BRC4 amino acid from valine to isoleucine (V1532I). However, the cancer-associated V1532F mutant exhibited very weak interaction with RAD51. This study used a comparative analysis of BRC4 between animal species to identify V1532 as an important residue that interacts with RAD51.
Volume 585(12)
Pages 1771-7
Published 2011-6-23
DOI 10.1016/j.febslet.2011.05.027
PII S0014-5793(11)00391-7
PMID 21601571
MeSH Amino Acid Substitution Animals BRCA2 Protein / metabolism* Humans Mutant Proteins Neoplasm Proteins Protein Binding / genetics Rad51 Recombinase / metabolism* Repetitive Sequences, Nucleic Acid* Two-Hybrid System Techniques Valine / metabolism*
IF 3.057
Times Cited 15
Human and Animal Cells HeLa(RCB0007)