| RRC ID |
18127
|
| 著者 |
Byambaa B, Konno T, Ishihara K.
|
| タイトル |
Cell adhesion control on photoreactive phospholipid polymer surfaces.
|
| ジャーナル |
Colloids Surf B Biointerfaces
|
| Abstract |
Non-invasive and effective cell recovery from culture substrates is important for the passage and characterization of cells. In this study, a photoreactive polymer surface, which uses UV-irradiation to control substrate cell adhesion, was prepared. The photoreactive phospholipid polymer (PMB-PL) reported herein, was composed of a both 2-methacryloyloxyethyl phosphorylcholine (MPC) unit as a cytocompatible unit and methacrylate bearing a photolabile nitrobenzyl group. The PMB-PL polymer was used to coat a cell culture substrate thus affording a photoreactive surface. Surface analysis of the PMB-PL coating indicated a strong photoresponse owing to the sensitivity of the PL unit. Before light exposure, the PMB-PL surface provided cell adhesion. Following UV-irradiation, the PMB-PL coating was converted to a neutral ζ-potential and hydrophilic surface. The photoreactive surface conversion process allowed for the detachment of adhered cells from the PMB-PL surface while maintaining cell viability. This study demonstrates the promise and significance of the PMB-PL photoreactive surface as a method to control cell attachment and detachment for cell function investigation.
|
| 巻・号 |
99
|
| ページ |
1-6
|
| 公開日 |
2012-11-1
|
| DOI |
10.1016/j.colsurfb.2011.08.029
|
| PII |
S0927-7765(11)00507-8
|
| PMID |
21982212
|
| MeSH |
Animals
Cell Adhesion / drug effects
Cell Culture Techniques
Cell Line
Cell Survival / drug effects
Coated Materials, Biocompatible / chemical synthesis*
Coated Materials, Biocompatible / pharmacology
Humans
Hydrogen-Ion Concentration
Hydrophobic and Hydrophilic Interactions
Magnetic Resonance Spectroscopy
Methacrylates / chemistry*
Mice
Microscopy, Electron, Scanning
Phosphorylcholine / analogs & derivatives*
Phosphorylcholine / chemistry
Photolysis
Polymers / chemical synthesis*
Polymers / pharmacology
Spectroscopy, Fourier Transform Infrared
Surface Properties
Ultraviolet Rays
|
| IF |
4.389
|
| 引用数 |
23
|
|
WOS 分野
|
BIOPHYSICS
CHEMISTRY, PHYSICAL
MATERIALS SCIENCE, BIOMATERIALS
|
| リソース情報 |
| ヒト・動物細胞 |
L929(RCB1451)
HeLa(RCB0007) |
