論文 - 詳細
| RRC ID | 18128 |
|---|---|
| 著者 | Li H, Solomon E, Duhachek Muggy S, Sun D, Zolkiewska A. |
| タイトル | Metalloprotease-disintegrin ADAM12 expression is regulated by Notch signaling via microRNA-29. |
| ジャーナル | J Biol Chem |
| Abstract |
Metalloprotease-disintegrin ADAM12 is overexpressed and frequently mutated in breast cancer. We report here that ADAM12 expression in cultured mammalian cells is up-regulated by Notch signals. Expression of a constitutively active form of Notch1 in murine fibroblasts, myoblasts, or mammary epithelial cells or activation of the endogenous Notch signaling by co-culture with ligand-expressing cells increases ADAM12 protein and mRNA levels. Up-regulation of ADAM12 expression by Notch requires new transcription, is activated in a CSL-dependent manner, and is abolished upon inhibition of IκB kinase. Expression of a constitutively active Notch1 in NIH3T3 cells increases the stability of Adam12 mRNA. We further show that the microRNA-29 family, which has a predicted conserved site in the 3'-untranslated region of mouse Adam12, plays a critical role in mediating the stimulatory effect of Notch on ADAM12 expression. In human cells, Notch up-regulates the expression of the long form, but not the short form, of ADAM12 containing a divergent 3'-untranslated mRNA region. These studies uncover a novel paradigm in Notch signaling and establish Adam12 as a Notch-related gene. |
| 巻・号 | 286(24) |
| ページ | 21500-10 |
| 公開日 | 2011-6-17 |
| DOI | 10.1074/jbc.M110.207951 |
| PII | S0021-9258(19)49031-5 |
| PMID | 21518768 |
| PMC | PMC3122209 |
| MeSH | 3' Untranslated Regions ADAM Proteins / biosynthesis* ADAM12 Protein Animals CHO Cells Cricetinae Cricetulus Gene Expression Regulation* Humans Membrane Proteins / biosynthesis* Mice MicroRNAs / biosynthesis* Models, Biological NIH 3T3 Cells Peptide Hydrolases / metabolism Receptor, Notch1 / metabolism Receptors, Notch / metabolism* Signal Transduction Up-Regulation |
| IF | 4.238 |
| 引用数 | 31 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | OT11(RCB1926) OT13(RCB1927) |