RRC ID 18130
Author Herlambang S, Kumagai M, Nomoto T, Horie S, Fukushima S, Oba M, Miyazaki K, Morimoto Y, Nishiyama N, Kataoka K.
Title Disulfide crosslinked polyion complex micelles encapsulating dendrimer phthalocyanine directed to improved efficiency of photodynamic therapy.
Journal J Control Release
Abstract Dendrimer phthalocyanine (DPc)-loaded polyion complex micelle (DPc/m) has been developed as photosensitizer (PS) formulation in photodynamic therapy (PDT). Incorporation of DPc into the micelle showed significant enhancement in the in vitro photocytotoxicity. Also, introduction of disulfide crosslinking in the micellar core further improved the in vitro PDT effect of DPc/m. Here, we aim to analyze the mechanism of the enhanced photocytotoxicity of DPc/m, particularly focusing on the photochemical reactions during photoirradiation. As a result, DPc/m has been shown to protect DPc from photobleaching induced by the reactions with serum proteins, although DPc were considerably quenched in the micellar core. Furthermore, the introduction of disulfide crosslinking into the micellar core has demonstrated to improve the efficiency of reactive oxygen species (ROS) production by DPc in the micellar core as well as more effectively prevent the photobleaching of DPc. These effects might lead to effective photochemical reactions by DPc/m, which may account for the enhanced photocytotoxicity. Our findings provide useful knowledge in designing PS formulations for effective PDT.
Volume 155(3)
Pages 449-57
Published 2011-11-7
DOI 10.1016/j.jconrel.2011.06.019
PII S0168-3659(11)00407-X
PMID 21704092
MeSH Cell Culture Techniques Cell Line, Tumor Cell Survival / drug effects Cell Survival / radiation effects Cross-Linking Reagents / chemistry* Dendrimers / administration & dosage* Dendrimers / chemistry Dendrimers / pharmacology Disulfides / chemistry* Drug Compounding Endocytosis Humans Indoles / administration & dosage* Indoles / chemistry Indoles / pharmacology Isoindoles Light Micelles Mitochondria / drug effects Mitochondria / metabolism Mitochondria / radiation effects Molecular Structure Photobleaching Photochemotherapy / methods* Photosensitizing Agents / administration & dosage* Photosensitizing Agents / chemistry Photosensitizing Agents / pharmacology Reactive Oxygen Species / metabolism Scattering, Radiation Spectrophotometry, Ultraviolet Surface Properties
IF 7.727
Times Cited 58
WOS Category PHARMACOLOGY & PHARMACY CHEMISTRY, MULTIDISCIPLINARY
Resource
Human and Animal Cells A549(RCB0098)