RRC ID 18137
Author Ma C, Zhang J, Fu J, Cheng L, Zhao G, Gu Y.
Title Up-regulation of VEGF by MC3T3-E1 cells treated with curculigoside.
Journal Phytother Res
Abstract Empirical evidence has shown that curculigoside, the main active compound of the traditionally used Chinese herb, Curculigo orchioides (Amaryllidaceae, rhizome), affects bone formation and fracture healing. However, the mechanistic details of these processes remain unclear. Therefore, the effects of curculigoside on immortalized, pre-osteoblastic mouse MC3T3-E1 cells was investigated. Following treatment with curculigoside, MC3T3-E1 cells exhibited an increased rate of proliferation. Higher levels of vascular endothelial growth factor (VEGF), Fms-like tyrosine kinase-1 (Flt-1) and bone morphogenetic protein-2 (BMP-2) were also detected in cell supernatants and cell lysates by ELISA and western blot analysis, respectively. Furthermore, the stimulatory effect of curculigoside was observed at relatively low doses (i.e. 10-100 μg/mL). In combination, these responses to treatment with curculigoside elucidate mechanistic details underlying the therapeutic effects of Curculigo orchioides on bone, and identifies these molecules as potential targets for the treatment of common metabolic bone diseases.
Volume 25(6)
Pages 922-6
Published 2011-6-1
DOI 10.1002/ptr.3449
PMID 21394809
MeSH 3T3 Cells Animals Benzoates / pharmacology* Bone Morphogenetic Protein 2 / biosynthesis Cell Growth Processes / drug effects Curculigo / chemistry Drugs, Chinese Herbal / pharmacology Glucosides / pharmacology* Mice Osteoblasts / cytology Osteoblasts / drug effects* Osteoblasts / metabolism Plant Extracts / pharmacology Rhizome / chemistry Up-Regulation / drug effects Vascular Endothelial Growth Factor A / biosynthesis* Vascular Endothelial Growth Factor A / metabolism Vascular Endothelial Growth Factor Receptor-1 / biosynthesis
IF 4.087
Times Cited 16
Human and Animal Cells MC3T3-E1(RCB1126)