論文 - 詳細
| RRC ID | 18176 |
|---|---|
| 著者 | Wei K, Li Y, Kim KO, Nakagawa Y, Kim BS, Abe K, Chen GQ, Kim IS. |
| タイトル | Fabrication of nano-hydroxyapatite on electrospun silk fibroin nanofiber and their effects in osteoblastic behavior. |
| ジャーナル | J Biomed Mater Res A |
| Abstract |
In this study, a novel tissue engineering scaffold material of electrospun silk fibroin/nano-hydroxyapatite (nHA) biocomposite was prepared by means of an effective calcium and phosphate (Ca-P) alternate soaking method. nHA was successfully produced on regenerated silk fibroin nanofiber as a substrate within several minutes without any pretreatments. The morphologies of both nonmineralized and mineralized nanofibers were analyzed using a field-emission scanning electron microscopy (FESEM). The crystallographic phases of the nHA were analyzed using X-ray diffraction (XRD). Fourier transform infrared (FTIR) spectrophotometer and thermogravimetry analyses (TGA) were employed to determine the type of functional groups and the amount of nHA presenting in the silk/nHA biocomposite nanofibers, respectively. The osteoblastic activities of this novel nanofibrous biocomposite scaffold were also investigated by employing osteoblastic-like MC3T3-E1 cell line. The cell functionality such as alkaline phosphatase (ALP) activity was ameliorated on mineralized nanofibers. All these results indicated that this silk/nHA biocomposite scaffold material may be a promising biomaterial for bone tissue engineering. |
| 巻・号 | 97(3) |
| ページ | 272-80 |
| 公開日 | 2011-6-1 |
| DOI | 10.1002/jbm.a.33054 |
| PMID | 21442728 |
| MeSH | 3T3 Cells Alkaline Phosphatase / metabolism Animals Bombyx / chemistry* Cell Adhesion Cell Proliferation Durapatite / chemistry* Durapatite / metabolism Fibroins / chemistry* Fibroins / metabolism Mice Nanofibers / chemistry* Nanofibers / ultrastructure Osteoblasts / cytology* Osteoblasts / metabolism Tissue Engineering / methods Tissue Scaffolds / chemistry* X-Ray Diffraction |
| IF | 3.525 |
| 引用数 | 97 |
| WOS 分野 | ENGINEERING, BIOMEDICAL MATERIALS SCIENCE, BIOMATERIALS |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | MC3T3-E1(RCB1126) |