| RRC ID |
18176
|
| 著者 |
Wei K, Li Y, Kim KO, Nakagawa Y, Kim BS, Abe K, Chen GQ, Kim IS.
|
| タイトル |
Fabrication of nano-hydroxyapatite on electrospun silk fibroin nanofiber and their effects in osteoblastic behavior.
|
| ジャーナル |
J Biomed Mater Res A
|
| Abstract |
In this study, a novel tissue engineering scaffold material of electrospun silk fibroin/nano-hydroxyapatite (nHA) biocomposite was prepared by means of an effective calcium and phosphate (Ca-P) alternate soaking method. nHA was successfully produced on regenerated silk fibroin nanofiber as a substrate within several minutes without any pretreatments. The morphologies of both nonmineralized and mineralized nanofibers were analyzed using a field-emission scanning electron microscopy (FESEM). The crystallographic phases of the nHA were analyzed using X-ray diffraction (XRD). Fourier transform infrared (FTIR) spectrophotometer and thermogravimetry analyses (TGA) were employed to determine the type of functional groups and the amount of nHA presenting in the silk/nHA biocomposite nanofibers, respectively. The osteoblastic activities of this novel nanofibrous biocomposite scaffold were also investigated by employing osteoblastic-like MC3T3-E1 cell line. The cell functionality such as alkaline phosphatase (ALP) activity was ameliorated on mineralized nanofibers. All these results indicated that this silk/nHA biocomposite scaffold material may be a promising biomaterial for bone tissue engineering.
|
| 巻・号 |
97(3)
|
| ページ |
272-80
|
| 公開日 |
2011-6-1
|
| DOI |
10.1002/jbm.a.33054
|
| PMID |
21442728
|
| MeSH |
3T3 Cells
Alkaline Phosphatase / metabolism
Animals
Bombyx / chemistry*
Cell Adhesion
Cell Proliferation
Durapatite / chemistry*
Durapatite / metabolism
Fibroins / chemistry*
Fibroins / metabolism
Mice
Nanofibers / chemistry*
Nanofibers / ultrastructure
Osteoblasts / cytology*
Osteoblasts / metabolism
Tissue Engineering / methods
Tissue Scaffolds / chemistry*
X-Ray Diffraction
|
| IF |
3.525
|
| 引用数 |
97
|
|
WOS 分野
|
ENGINEERING, BIOMEDICAL
MATERIALS SCIENCE, BIOMATERIALS
|
| リソース情報 |
| ヒト・動物細胞 |
MC3T3-E1(RCB1126) |
