論文 - 詳細
| RRC ID | 18182 |
|---|---|
| 著者 | Nagahama M, Ohkubo A, Oda M, Kobayashi K, Amimoto K, Miyamoto K, Sakurai J. |
| タイトル | Clostridium perfringens TpeL glycosylates the Rac and Ras subfamily proteins. |
| ジャーナル | Infect Immun |
| Abstract |
Clostridium perfringens TpeL belongs to a family of large clostridial cytotoxins that encompasses Clostridium difficile toxin A (TcdA) and B (TcdB) and Clostridium sordellii lethal toxin (TcsL). We report here the identification of the TpeL-catalyzed modification of small GTPases. A recombinant protein (TpeL1-525) derived from the TpeL N-terminal catalytic domain in the presence of streptolysin O (SLO) induced the rounding of Vero cells and the glycosylation of cellular Rac1. Among several hexoses tested, UDP-N-acetyl-glucosamine (UDP-GlcNAc) and UDP-glucose (UDP-Glc) served as cosubstrates for TpeL1-525-catalyzed modifications. TpeL1-525 catalyzed the incorporation of UDP-Glc into Ha-Ras, Rap1B, and RalA and of UDP-GlcNAc into Rac1, Ha-Ras, Rap1B, and RalA. In Rac1, TpeL and TcdB share the same acceptor amino acid for glycosylation, Thr-35. In Vero cells treated with TpeL1-525 in the presence of SLO, glycosylation leads to a translocation of the majority of Rac1 and Ha-Ras to the membrane. We demonstrate for first time that TpeL uses both UDP-GlcNAc and UDP-Glc as donor cosubstrates and modifies the Rac1 and Ras subfamily by glycosylation to mediate its cytotoxic effects. |
| 巻・号 | 79(2) |
| ページ | 905-10 |
| 公開日 | 2011-2-1 |
| DOI | 10.1128/IAI.01019-10 |
| PII | IAI.01019-10 |
| PMID | 21098103 |
| PMC | PMC3028844 |
| MeSH | Animals Bacterial Toxins / metabolism* Chlorocebus aethiops Clostridium perfringens / genetics Clostridium perfringens / metabolism* Cytotoxins / metabolism* GTP Phosphohydrolases / metabolism Glycosylation Protein Binding Proto-Oncogene Proteins c-akt / metabolism* Recombinant Proteins Substrate Specificity Vero Cells ras Proteins / metabolism* |
| IF | 3.201 |
| 引用数 | 34 |
| WOS 分野 | INFECTIOUS DISEASES IMMUNOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | Vero(RCB0001) |