論文 - 詳細
| RRC ID | 18184 |
|---|---|
| 著者 | Ukaji T, Iwasa MA, Kai O. |
| タイトル | Establishment of gerbil-mouse heterohybridoma secreting immunoglobulin of Mongolian gerbil (Meriones unguiculatus) establishment of gerbil-mouse heterohybridoma. |
| ジャーナル | J Immunol Methods |
| Abstract |
Hybridomas, generated by fusing myeloma cells with B lymphocytes, secrete monoclonal antibodies (mAbs) specific for an antigen. To establish hybridomas that secrete Mongolian gerbil (Meriones unguiculatus) mAbs, we immunized gerbils with keyhole limpet hemocyanin (KLH) and fused splenocytes from them with a non-secreting mouse myeloma cell line (P3-X63-Ag8.653). We obtained hybridomas that secreted immunoglobulin (Ig) against KLH. These hybridomas had more chromosomes than either parent and retained several gerbil chromosomes. Therefore, these cells were heterohybridomas with both gerbil and mouse chromosomes. They expressed gerbil Ig heavy-chain constant (IGHC) region mRNA, but not mouse gamma (γ) 1 IGHC. SDS-PAGE and Western blot analyses indicated that the cells secreted whole Ig molecules of gerbil origin. Moreover, the cells continued secreting Ig for several months. |
| 巻・号 | 373(1-2) |
| ページ | 174-80 |
| 公開日 | 2011-10-28 |
| DOI | 10.1016/j.jim.2011.08.015 |
| PII | S0022-1759(11)00220-1 |
| PMID | 21907207 |
| MeSH | Animals Blotting, Western Cell Fusion Cell Line, Tumor Chromosomes, Mammalian / genetics Electrophoresis, Polyacrylamide Gel Female Gerbillinae / genetics Gerbillinae / immunology* Hemocyanins / immunology Hybridomas / immunology* Hybridomas / metabolism Immunization Immunoglobulin A / genetics Immunoglobulin A / immunology Immunoglobulin G / genetics Immunoglobulin G / immunology Immunoglobulin Heavy Chains / genetics Immunoglobulin Heavy Chains / immunology Immunoglobulin M / genetics Immunoglobulin M / immunology Immunoglobulins / genetics Immunoglobulins / immunology* Karyotyping Lymphocytes / cytology Lymphocytes / immunology Lymphocytes / metabolism Male Mice / genetics Mice / immunology* Mice, Inbred ICR Multiple Myeloma / genetics Multiple Myeloma / immunology Multiple Myeloma / pathology Reverse Transcriptase Polymerase Chain Reaction |
| IF | 1.901 |
| 引用数 | 5 |
| WOS 分野 | BIOCHEMICAL RESEARCH METHODS IMMUNOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | P3-X63-Ag8.653(RCB0146) |