| RRC ID |
18192
|
| Author |
Seong AR, Yoo JY, Choi K, Lee MH, Lee YH, Lee J, Jun W, Kim S, Yoon HG.
|
| Title |
Delphinidin, a specific inhibitor of histone acetyltransferase, suppresses inflammatory signaling via prevention of NF-κB acetylation in fibroblast-like synoviocyte MH7A cells.
|
| Journal |
Biochem Biophys Res Commun
|
| Abstract |
Histone acetyltransferase (HAT) inhibitors (HATi) isolated from dietary compounds have been shown to suppress inflammatory signaling, which contributes to rheumatoid arthritis. Here, we identified a novel HATi in Punica granatum L. known as delphinidin (DP). DP did not affect the activity of other epigenetic enzymes (histone deacetylase, histone methyltransferase, or sirtuin1). DP specifically inhibited the HAT activities of p300/CBP. It also inhibited p65 acetylation in MH7A cells, a human rheumatoid arthritis synovial cell line. DP-induced hypoacetylation was accompanied by cytosolic accumulation of p65 and nuclear localization of IKBα. Accordingly, DP treatment inhibited TNFα-stimulated increases in NF-κB function and expression of NF-κB target genes in these cells. Importantly, DP suppressed lipopolysaccharide-induced pro-inflammatory cytokine expression in Jurkat T lymphocytes, demonstrating that HATi efficiently suppresses cytokine-mediated immune responses. Together, these results show that the HATi activity of DP counters anti-inflammatory signaling by blocking p65 acetylation and that this compound may be useful in preventing inflammatory arthritis.
|
| Volume |
410(3)
|
| Pages |
581-6
|
| Published |
2011-7-8
|
| DOI |
10.1016/j.bbrc.2011.06.029
|
| PII |
S0006-291X(11)00987-9
|
| PMID |
21683061
|
| MeSH |
Acetylation / drug effects
Anthocyanins / pharmacology*
Anti-Inflammatory Agents, Non-Steroidal / pharmacology*
Arthritis / genetics
Arthritis / immunology*
Cell Line
Cytokines / antagonists & inhibitors*
Enzyme Inhibitors / pharmacology*
Fibroblasts / drug effects
Fibroblasts / metabolism
Gene Expression / drug effects
Gene Expression / genetics
Histone Acetyltransferases / antagonists & inhibitors*
Humans
Jurkat Cells
NF-kappa B / metabolism*
Synovial Fluid / drug effects
Synovial Fluid / metabolism
|
| IF |
2.985
|
| Times Cited |
45
|
|
WOS Category
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Human and Animal Cells |
MH7A(RCB1512) |
