RRC ID 18194
Author Koh DJ, Ahn HS, Chung HS, Lee H, Kim Y, Lee JY, Kim DG, Hong M, Shin M, Bae H.
Title Inhibitory effects of casticin on migration of eosinophil and expression of chemokines and adhesion molecules in A549 lung epithelial cells via NF-κB inactivation.
Journal J Ethnopharmacol
Abstract ETHNOPHARMACOLOGICAL RELEVANCE:The fruits of Vitex rotundifolia L. have long been used for the treatment of inflammation of the respiratory tract in East Asia.
AIM:To determine if casticin, one of the constituents of Vitex rotundifolia L., has anti-allergic and anti-inflammatory effects in asthma.
MATERIALS AND METHODS:The in vitro anti-inflammatory activity of casticin was studied in A549 human type II-like epithelial lung cells using an eotaxin inhibition assay. Additionally, its effects on eotaxin, regulated on activation normal T cell expressed and secreted (RANTES), vascular cell adhesion molecule (VCAM)-1, and inter-cellular adhesion molecule (ICAM)-1 expression were investigated by real time-polymerase chain reaction (real time-PCR). The inhibition of nuclear factor κB (NF-κB) activity in the presence of casticin was determined by analyzing confocal microscopy images of fluorescence immunocytochemical analysis while the suppression of inhibitory κB (IκB)-α phosphorylation was studied using Western blot analysis. Finally, the inhibitory effect of casticin on eosinophil migration toward prestimulated A549 cell media was measured using the human eosinophilic leukemia cell line.
RESULTS AND DISCUSSION:Casticin significantly suppressed eotaxin production in cytokine activated A549 lung epithelial cells. Casticin also suppressed the mRNA expression levels of eotaxin, RANTES, VCAM-1, and ICAM-1, which subsequently contributed to the inhibition of eosinophil migration. Furthermore, casticin inhibited IκB-α phosphorylation and nuclear translocation of p65 in A549 cells.
CONCLUSION:Casiticin inhibited the eosinophil migration and activity of chemokines and adhesion molecules involved in the inflammatory process of asthma by suppressing the NF-κB pathway. These results suggest that casticin has the potential for use in the treatment of allergic asthma.
Volume 136(3)
Pages 399-405
Published 2011-7-14
DOI 10.1016/j.jep.2011.01.014
PII S0378-8741(11)00015-8
PMID 21251967
MeSH Anti-Inflammatory Agents / pharmacology Anti-Inflammatory Agents / therapeutic use Asthma / drug therapy* Asthma / metabolism Cell Adhesion Molecules / metabolism* Cell Line Chemokines, CC / biosynthesis* Chemokines, CC / genetics Chemotaxis, Leukocyte / drug effects Cytokines / metabolism Eosinophils / drug effects* Eosinophils / metabolism Epithelial Cells / drug effects Flavonoids / pharmacology Flavonoids / therapeutic use* Fruit Humans I-kappa B Proteins / metabolism Lung / drug effects Lung / metabolism NF-KappaB Inhibitor alpha NF-kappa B / antagonists & inhibitors Phytotherapy* Plant Extracts / pharmacology Plant Extracts / therapeutic use RNA, Messenger / metabolism Transcription Factor RelA / metabolism Vitex / chemistry*
IF 3.414
Times Cited 25
Human and Animal Cells EoL-1 cell (RCB0641)