RRC ID 18196
Author Takeuchi N, Machigashira M, Yamashita D, Shirakata Y, Kasuga T, Noguchi K, Ban S.
Title Cellular compatibility of a gamma-irradiated modified siloxane-poly(lactic acid)-calcium carbonate hybrid membrane for guided bone regeneration.
Journal Dent Mater J
Abstract A bi-layered silicon-releasable membrane consisting of a siloxane-poly(lactic acid) (PLA)-vaterite hybrid material (Si-PVH) microfiber mesh and a PLA microfiber mesh has been developed by an electrospinning method for guided bone regeneration (GBR) application. The bi-layered membrane was modified to a three-laminar structure by sandwiching an additional PLA microfiber mesh between the Si-PVH and PLA microfiber meshes (Si-PVH/PLA membrane). In this study, the influence of gamma irradiation, used for sterilization, on biological properties of the Si-PVH/PLA membrane was evaluated with osteoblasts and fibroblasts. After gamma irradiation, while the average molecular weight of the Si-PVH/PLA membrane decreased, the Si-PVH/PLA membrane promoted cell proliferation and differentiation (alkaline phosphatase activity and calcification) of osteoblasts, compared with the poly(lactide-co-glycolide) membrane. These results suggest that the gamma-irradiated Si-PVH/PLA membrane is biocompatible with both fibroblasts and osteoblasts, and may have an application for GBR.
Volume 30(5)
Pages 730-8
Published 2011
DOI 10.4012/dmj.2011-075
PII JST.JSTAGE/dmj/2011-075
PMID 21946495
MeSH 3T3 Cells Alkaline Phosphatase / analysis Animals Biocompatible Materials / chemistry* Biocompatible Materials / radiation effects Bone Regeneration / physiology* Calcification, Physiologic / physiology Calcium Carbonate / chemistry* Calcium Carbonate / radiation effects Cell Culture Techniques Cell Differentiation / physiology Cell Proliferation Cells, Cultured Equipment Design Fibroblasts / physiology Gamma Rays Gingiva / cytology Guided Tissue Regeneration / instrumentation* Humans Lactic Acid / chemistry* Lactic Acid / radiation effects Materials Testing Membranes, Artificial* Mice Microscopy, Electron, Scanning Molecular Weight Osteoblasts / physiology Polyesters Polyglycolic Acid / chemistry Polyglycolic Acid / radiation effects Polylactic Acid-Polyglycolic Acid Copolymer Polymers / chemistry* Polymers / radiation effects Siloxanes / chemistry* Siloxanes / radiation effects Sterilization / methods Surgical Mesh X-Ray Diffraction
IF 1.424
Times Cited 6
Human and Animal Cells MC3T3-E1 (RCB1126)