論文 - 詳細
| RRC ID | 1820 |
|---|---|
| 著者 | Sakudoh T, Sezutsu H, Nakashima T, Kobayashi I, Fujimoto H, Uchino K, Banno Y, Iwano H, Maekawa H, Tamura T, Kataoka H, Tsuchida K. |
| タイトル | Carotenoid silk coloration is controlled by a carotenoid-binding protein, a product of the Yellow blood gene. |
| ジャーナル | Proc Natl Acad Sci U S A |
| Abstract |
Mechanisms for the uptake and transport of carotenoids, essential nutrients for humans, are not well understood in any animal system. The Y (Yellow blood) gene, a critical cocoon color determinant in the silkworm Bombyx mori, controls the uptake of carotenoids into the intestinal mucosa and the silk gland. Here we provide evidence that the Y gene corresponds to the intracellular carotenoid-binding protein (CBP) gene. In the Y recessive strain, the absence of an exon, likely due to an incorrect mRNA splicing caused by a transposon-associated genomic deletion, generates a nonfunctional CBP mRNA, resulting in colorless hemolymph and white cocoons. Enhancement of carotenoid uptake and coloration of the white cocoon was achieved by germ-line transformation with the CBP gene. This study demonstrates the existence of a genetically facilitated intracellular process beyond passive diffusion for carotenoid uptake in the animal phyla, and paves the way for modulating silk color and lipid content through genetic engineering. |
| 巻・号 | 104(21) |
| ページ | 8941-6 |
| 公開日 | 2007-5-22 |
| DOI | 10.1073/pnas.0702860104 |
| PII | 0702860104 |
| PMID | 17496138 |
| PMC | PMC1885607 |
| MeSH | Alleles Animals Animals, Genetically Modified Bombyx / genetics Bombyx / metabolism Carotenoids / metabolism* Carrier Proteins / genetics Carrier Proteins / metabolism* Color Gene Expression Regulation Genome, Insect / genetics Insect Proteins / genetics Insect Proteins / metabolism* Molecular Sequence Data Phenotype Silk / metabolism* |
| IF | 9.412 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Wikipedia |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 情報 | Silkworm |