| RRC ID |
18213
|
| 著者 |
Teramura T, Takehara T, Onodera Y, Nakagawa K, Hamanishi C, Fukuda K.
|
| タイトル |
Mechanical stimulation of cyclic tensile strain induces reduction of pluripotent related gene expressions via activation of Rho/ROCK and subsequent decreasing of AKT phosphorylation in human induced pluripotent stem cells.
|
| ジャーナル |
Biochem Biophys Res Commun
|
| Abstract |
Mechanical stimulation has been shown to regulate the proliferation and differentiation of stem cells. However, the effects of the mechanical stress on the stemness or related molecular mechanisms have not been well determined. Pluripotent stem cells such as embryonic stem (ES) cells and induced pluripotent stem (iPS) cells are used as good materials for cell transplantation therapy and research of mammalian development, since they can self-renew infinitely and differentiate into various cell lineages. Here we demonstrated that the mechanical stimulation to human iPS cells altered alignment of actin fibers and expressions of the pluripotent related genes Nanog, POU5f1 and Sox2. In the mechanically stimulated iPS cells, small GTPase Rho was activated and interestingly, AKT phosphorylation was decreased. Inhibition of Rho-associated kinase ROCK recovered the AKT phosphorylation and the gene expressions. These results clearly suggested that the Rho/ROCK is a potent primary effector of mechanical stress in the pluripotent stem cells and it participates to pluripotency-related signaling cascades as an upper stream regulator.
|
| 巻・号 |
417(2)
|
| ページ |
836-41
|
| 公開日 |
2012-1-13
|
| DOI |
10.1016/j.bbrc.2011.12.052
|
| PII |
S0006-291X(11)02261-3
|
| PMID |
22206673
|
| MeSH |
Cells, Cultured
Gene Expression Regulation*
Homeodomain Proteins / genetics
Humans
Induced Pluripotent Stem Cells / enzymology
Induced Pluripotent Stem Cells / physiology*
Mechanotransduction, Cellular / genetics*
Nanog Homeobox Protein
Octamer Transcription Factor-3 / genetics
Phosphorylation
Proto-Oncogene Proteins c-akt / metabolism
SOXB1 Transcription Factors / genetics
Tensile Strength
rho-Associated Kinases / genetics
rho-Associated Kinases / metabolism*
|
| IF |
2.985
|
| 引用数 |
24
|
|
WOS 分野
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
253G1(HPS0002) |
