RRC ID 18220
著者 Tamura M, Sato MM, Nashimoto M.
タイトル Regulation of CXCL12 expression by canonical Wnt signaling in bone marrow stromal cells.
ジャーナル Int J Biochem Cell Biol
Abstract CXCL12 (stromal cell-derived factor-1, SDF-1), produced by stromal and endothelial cells including cells of the bone marrow, binds to its receptor CXCR4 and this axis regulates hematopoietic cell trafficking. Recently, osteoclast precursor cells were found to express CXCR4 and a potential role for the CXCL12-CXCR4 axis during osteoclast precursor cell recruitment/retention and development was proposed as a regulator of bone resorption. We examined the role of canonical Wnt signaling in regulating the expression of CXCL12 in bone marrow stromal cells. In mouse stromal ST2 cells, CXCL12 mRNA was expressed, while its expression was reduced in Wnt3a over-expressing ST2 (Wnt3a-ST2) cells or by treatment with lithium chloride (LiCl). Wnt3a decreased CXCL12 levels in culture supernatants from mouse bone marrow stromal cells. The culture supernatant from Wnt3a-ST2 cells also reduced migratory activity of bone marrow-derived cells in a Transwell migration assay. Silencing of glycogen synthase kinase-3β decreased CXCL12 expression, suggesting that the canonical Wnt signaling pathway regulates CXCL12 expression. In a transfection assay, LiCl down-regulated the activity of a reporter gene, a 1.8kb fragment of the 5'-flanking region of the CXCL12 gene. These results show that canonical Wnt signaling regulates CXCL12 gene expression at the transcriptional level, and this is the first study linking chemokine expression to canonical Wnt signaling.
巻・号 43(5)
ページ 760-7
公開日 2011-5-1
DOI 10.1016/j.biocel.2011.01.021
PII S1357-2725(11)00038-0
PMID 21296678
MeSH Animals Bone Marrow Cells / cytology* Cell Line Cell Movement Chemokine CXCL12 / biosynthesis Chemokine CXCL12 / genetics* Down-Regulation Gene Expression Regulation* Gene Knockdown Techniques Glycogen Synthase Kinase 3 / deficiency Glycogen Synthase Kinase 3 / genetics Glycogen Synthase Kinase 3 beta Mice Osteoclasts / cytology Promoter Regions, Genetic / genetics Signal Transduction* Stromal Cells / cytology Stromal Cells / metabolism Wnt Proteins / metabolism* Wnt3 Protein Wnt3A Protein
IF 3.673
引用数 35
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
リソース情報
ヒト・動物細胞 ST2(RCB0224)