| RRC ID |
18233
|
| Author |
Yamawaki H, Kuramoto J, Kameshima S, Usui T, Okada M, Hara Y.
|
| Title |
Omentin, a novel adipocytokine inhibits TNF-induced vascular inflammation in human endothelial cells.
|
| Journal |
Biochem Biophys Res Commun
|
| Abstract |
Omentin is a recently identified adipocytokine with insulin-sensitizing effect. While lack of omentin may be related to the pathogenesis of obesity-related cardiovascular diseases, its effect in vasculature is largely unknown. We examined effects of omentin on vascular endothelial inflammatory states. Western blotting was performed to analyze inflammatory signal transduction in cultured vascular endothelia cells. The cyclic guanosine monophosphate (cGMP) content was measured using enzyme immunoassay. Treatment of human umbilical vein endothelial cells with omentin (300 ng/ml, 20 min) induced phosphorylation of 5'-AMP-activated protein kinase (AMPK) (Thr 172) and endothelial nitric oxide (NO) synthase (eNOS) (Ser 1177). Consistently, omentin increased the cGMP level. Pretreatment with omentin (300 ng/ml, 30 min) significantly inhibited the phosphorylation of JNK as well as expression of cyclooxygenase (COX)-2 by TNF-α (5 ng/ml, 20 min-24h). An inhibitor of JNK significantly inhibited the TNF-α-induced COX-2 expression. Inhibitory effect of omentin on TNF-α-induced COX-2 was reversed by a NOS inhibitor. The present results demonstrate for the first time that omentin plays an anti-inflammatory role by preventing the TNF-α-induced COX-2 expression in vascular endothelial cells. Omentin inhibits COX-2 induction via preventing the JNK activation presumably through activation of AMPK/eNOS/NO pathways.
|
| Volume |
408(2)
|
| Pages |
339-43
|
| Published |
2011-5-6
|
| DOI |
10.1016/j.bbrc.2011.04.039
|
| PII |
S0006-291X(11)00613-9
|
| PMID |
21514279
|
| MeSH |
Adipokines / genetics
Adipokines / pharmacology
Adipokines / physiology*
Cells, Cultured
Cyclooxygenase 2 / metabolism
Cytokines / pharmacology
Cytokines / physiology*
Endothelium, Vascular / drug effects
Endothelium, Vascular / metabolism*
GPI-Linked Proteins / pharmacology
GPI-Linked Proteins / physiology
Humans
Lectins / pharmacology
Lectins / physiology*
Nitric Oxide Synthase Type III / metabolism
Phosphorylation
Tumor Necrosis Factor-alpha / pharmacology
Vasculitis / metabolism*
|
| IF |
2.985
|
| Times Cited |
169
|
|
WOS Category
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Human and Animal Cells |
U-937 DE-4(RCB0435) |
