RRC ID 183
Author Oriyanhan W, Yamazaki K, Miwa S, Takaba K, Ikeda T, Komeda M.
Title Taurine prevents myocardial ischemia/reperfusion-induced oxidative stress and apoptosis in prolonged hypothermic rat heart preservation.
Journal Heart Vessels
Abstract Taurine (2-aminoethanesulfonic acid) is a potent antioxidant and inhibits cell apoptosis in ischemic reperfusion injury. In this study we evaluated whether addition of taurine to St. Thomas' cardioplegic solution enhances its myocardial protective effects in prolonged hypothermic heart preservation in rats. Hearts isolated from male Sprague-Dawley rats were mounted on a Langendorff apparatus to estimate baseline cardiac function, then arrested and stored in St. Thomas' cardioplegic solution, with taurine (10 mM; taurine group, n = 8) or without taurine (control group, n = 8), for 6 h at 4 degrees C. After storage, the hearts were reperfused and heart rate (HR), coronary flow (CF), left ventricular developed pressure (LVP), and positive maximum left ventricular developing pressure (max LV dp/dt) were measured. The LV tissue was examined immunohistochemically for determining DNA oxidative stress and cell apoptosis. Compared with control groups, recovery of LVP (P < 0.001), max LV dp/dt (P < 0.001), and coronary flow (P < 0.001) were significantly enhanced, whereas glutamic oxaloacetic transaminase (P < 0.01), lactate dehydrogenase (P < 0.05), creatine phosphate kinase (P < 0.01), 8-hydroxy-2'-deoxyguanosine index (P < 0.01), caspase-3 mRNA expression (P < 0.05), and percentage of TUNEL-positive cardiomyocytes (P < 0.05) were reduced in the taurine group. Addition of taurine to St. Thomas' cardioplegic solution improved cardiac function recovery for prolonged hypothermic rat heart preservation by suppressing DNA oxidative stress and cell apoptosis.
Volume 20(6)
Pages 278-85
Published 2005-11-1
DOI 10.1007/s00380-005-0841-9
PMID 16314910
MeSH Animals Apoptosis / drug effects* Apoptosis / physiology Coronary Circulation / drug effects Coronary Circulation / physiology Cryopreservation* Graft Survival / drug effects Graft Survival / physiology Heart Transplantation / pathology Heart Transplantation / physiology* Lipid Peroxidation / drug effects Lipid Peroxidation / physiology Male Myocardial Reperfusion Injury / pathology Myocardial Reperfusion Injury / physiopathology Myocardial Reperfusion Injury / prevention & control* Myocardium / pathology Organ Preservation* Oxidative Stress / drug effects* Oxidative Stress / physiology Rats Rats, Sprague-Dawley Reactive Oxygen Species / metabolism Taurine / pharmacology* Ventricular Function, Left / drug effects Ventricular Function, Left / physiology
IF 1.618
Times Cited 56