RRC ID 18542
Author Chen J, Qin S, Xiao J, Tanigawa S, Uto T, Hashimoto F, Fujii M, Hou DX.
Title A genome-wide microarray highlights the antiinflammatory genes targeted by oolong tea theasinensin A in macrophages.
Journal Nutr Cancer
Abstract Theasinensin A is one of the oolong tea theasinensins, which differ from green tea catechins and black tea theaflavins. In a previous study, we found that theasinesin A had a potential effect on antiinflammation since theasinensin A suppressed LPS-induced COX2 and PGE(2) production. To clarify the molecular mechanisms, we investigated the gene expression profiling in macrophage-like cells treated with theasinensin A through a genome-wide DNA microarray in the present study. Among 22,050 oligonucleotides, the expression levels of 406 genes were increased by ≥3-fold in LPS-activated RAW264 cells, 259 gene signals of which were attenuated by theasinensin A treatment (≥2-fold). Expression levels of 717 genes were decreased by ≥3-fold in LPS-activated cells, of which 471 gene signals were restored by theasinensin A treatment (≥2-fold). These genes were further categorized as "defense, inflammatory response, cytokines activities, and receptor activities," and some of them were confirmed by real-time polymerase chain reaction. Furthermore, pathways analysis revealed that theasinensin A regulated the relevant expression networks of chemokines, interleukins, and interferons to exert its antiinflammatory effects.
Volume 63(7)
Pages 1064-73
Published 2011
DOI 10.1080/01635581.2011.596643
PMID 21864060
MeSH Animals Anti-Inflammatory Agents / pharmacology Benzopyrans / pharmacology* Cell Line Chemokines / genetics Chemokines / metabolism Gene Expression Profiling Gene Expression Regulation / drug effects* Interferons / genetics Interferons / metabolism Interleukins / genetics Interleukins / metabolism Lipopolysaccharides / adverse effects Macrophages / cytology Macrophages / drug effects* Macrophages / metabolism Mice Oligonucleotide Array Sequence Analysis* Phenols / pharmacology* Real-Time Polymerase Chain Reaction Reverse Transcriptase Polymerase Chain Reaction Signal Transduction Tea / chemistry*
IF 2.261
Times Cited 4
Human and Animal Cells RAW 264 (RCB0535)