| RRC ID |
18547
|
| Author |
Arena F, Singh JB, Gianolio E, Stefanìa R, Aime S.
|
| Title |
β-Gal gene expression MRI reporter in melanoma tumor cells. Design, synthesis, and in vitro and in vivo testing of a Gd(III) containing probe forming a high relaxivity, melanin-like structure upon β-Gal enzymatic activation.
|
| Journal |
Bioconjug Chem
|
| Abstract |
The aim of this work is to design and test an MRI probe (Gd-DOTAtyr-gal) able to report on the gene expression of β-galactosidase (β-Gal) in melanoma cells. The probe consists of a Gd-DOTA reporter bearing on its surface a tyrosine-galactose-pyranose functionality that, upon the release of the sugar moiety, readily transforms, in the presence of tyrosinase, into melanin oligomeric/polymeric mixture. The formation of Gd-DOTA-containing melanin oligomers and polymers is accompanied by a marked increase of the water proton relaxation rate. The steps involving the release of the galactose-pyranose group and the formation of the melanin-like structure have been carefully investigated in vitro by relaxometric and UV-vis measurements. Cellular uptake studies of Gd-DOTAtyr-gal by melanoma cells have shown that the probe enters the cells, and it appears not to be confined in endosomal vesicles. Using B16-F10LacZ transfected cells, the fast formation of paramagnetic melanin-Gd(III)-containing species has been assessed by the measurement of increased longitudinal relaxation rates of the cellular pellets suspensions. The in vitro results have been confirmed in in vivo MRI investigations on murine melanoma tumor bearing mice. Upon direct injection of Gd-DOTAtyr-gal, a good contrast is observed after 5 h post injection in B16-F10LacZ tumors, but not in B16-F10 tumors lacking the β-Gal enzyme. Gd-DOTAtyr-gal in combination with tyrosinase introduces a novel approach for the detection of β-Gal expression by MRI in vivo.
|
| Volume |
22(12)
|
| Pages |
2625-35
|
| Published |
2011-12-21
|
| DOI |
10.1021/bc200486j
|
| PMID |
22035020
|
| MeSH |
Animals
Cell Line, Tumor
Enzyme Activation
Female
Gene Expression Regulation, Neoplastic
Heterocyclic Compounds* / chemistry
Heterocyclic Compounds* / metabolism
Magnetic Resonance Imaging / methods*
Melanoma / enzymology*
Melanoma / genetics
Mice
Mice, Inbred C57BL
Monophenol Monooxygenase / metabolism
Organometallic Compounds* / chemistry
Organometallic Compounds* / metabolism
beta-Galactosidase / genetics
beta-Galactosidase / metabolism*
|
| IF |
4.031
|
| Times Cited |
37
|
|
WOS Category
|
BIOCHEMICAL RESEARCH METHODS
CHEMISTRY, ORGANIC
BIOCHEMISTRY & MOLECULAR BIOLOGY
CHEMISTRY, MULTIDISCIPLINARY
|
| Resource |
| Human and Animal Cells |
B16 melanoma/lacZ(RCB1284) |
